Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation at 1 mg/ml | Escherichia coli | |
Phospholipids | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic cooperativity towards glycerol 3-phosphate and palmitoyl-CoA | Escherichia coli | |
0.0046 | - |
palmitoyl-CoA | strain 8, reconstituted enzyme | Escherichia coli | |
0.0056 | - |
palmitoyl-CoA | strain 8, membrane-associated enzyme | Escherichia coli | |
0.006 | - |
palmitoyl-CoA | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.0078 | - |
palmitoyl-CoA | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.012 | - |
palmitoyl-CoA | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme | Escherichia coli | |
0.017 | - |
palmitoyl-CoA | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme | Escherichia coli | |
0.102 | - |
sn-glycerol 3-phosphate | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.105 | - |
sn-glycerol 3-phosphate | strain 8, membrane-associated enzyme | Escherichia coli | |
0.108 | - |
sn-glycerol 3-phosphate | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.79 | - |
sn-glycerol 3-phosphate | strain 8, reconstituted enzyme | Escherichia coli | |
0.92 | - |
sn-glycerol 3-phosphate | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme | Escherichia coli | |
1.1 | - |
sn-glycerol 3-phosphate | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Escherichia coli | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
different molecular masses depending on Triton X-100 concentration | Escherichia coli |
88000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4 | - |
Escherichia coli | - |
K-12 derivative strains 8, wild-type genotype | - |
Purification (Comment) | Organism |
---|---|
partial purification | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution in cardiolipin vesicles | Escherichia coli |
reconstitution in vesicles | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 4 | reconstituted enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Escherichia coli |
More | monomer or oligomer, depending on Triton X-100 concentration | Escherichia coli |