Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asolectin | i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme | Rattus norvegicus | |
bovine serum albumin | - |
Rattus norvegicus | |
phosphatidylcholine | - |
Rattus norvegicus | |
phosphatidylserine | activation of mitochondrial enzyme | Rattus norvegicus | |
Phospholipids | - |
Rattus norvegicus |
General Stability | Organism |
---|---|
asolectin stabilizes cholate-containing preparations | Rattus norvegicus |
bovine serum albumin stabilizes | Rattus norvegicus |
cholate inactivates during storage | Rattus norvegicus |
repeated freezing and thawing inactivates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-palmitoyl-sn-glycerol 3-phosphate | - |
Rattus norvegicus | |
cardiolipin | - |
Rattus norvegicus | |
cholate | - |
Rattus norvegicus | |
diacylglycerophosphate | - |
Rattus norvegicus | |
Monoacylglycerophosphate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | membrane-bound | Rattus norvegicus | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation of microsomal and mitochondrial enzyme | Rattus norvegicus | |
Co2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
mitochondrial enzyme, cholate, ammonium sulfate, partial purification | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0138 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 2 months after the removal of cholate | Rattus norvegicus |
-70°C, 35% loss of activity after 2 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | no acyl-donors are hexanoyl-CoA, octanoyl-CoA | Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + sn-glycerol 3-phosphate | 2% of activity with palmitoyl-CoA | Rattus norvegicus | CoA + 1-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
myristoyl-CoA + sn-glycerol 3-phosphate | i.e. tetradecanoyl-CoA | Rattus norvegicus | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
n-decanoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-n-decanoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | 7% of activity with palmitoyl-CoA | Rattus norvegicus | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | poor substrate | Rattus norvegicus | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |