Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(3-Morpholino)propanesulfonic acid-NaOH | i.e. MOPS-NaOH, activation, up to 30 mM | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
sn-glycerol 3-phosphate | isoforms 1 and 2 | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | soluble | Spinacia oleracea | 9507 | - |
chloroplast | soluble | Pisum sativum | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by divalent ions | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 soluble chloroplast isoforms, gel filtration | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
pea, var. kleine Rheinländerin | - |
Spinacia oleracea | - |
var. Vital | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
2 isoforms of different pI-values | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
leaf | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
- |
Spinacia oleracea |
1.74 | - |
isoform 1 | Pisum sativum |
3.2 | - |
isoform 2 | Pisum sativum |
Storage Stability | Organism |
---|---|
-20°C, 50% w/v glycerol, 3 years, no loss of activity | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | chloroplastic enzyme has no strict acyl-CoA specificity | Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | chloroplastic enzyme has no strict acyl-CoA specificity | Pisum sativum | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | specific for glycerol 3-phosphate | Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | specific for glycerol 3-phosphate | Pisum sativum | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Pisum sativum | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | dihydroxyacetone phosphate is ineffective as acyl-acceptor | Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | dihydroxyacetone phosphate is ineffective as acyl-acceptor | Pisum sativum | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
linolenoyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-linolenoyl-sn-glycerol 3-phosphate | - |
? | |
linolenoyl-CoA + sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-linolenoyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | preferred substrate | Pisum sativum | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | preferred in an equimolar mixture of palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA | Spinacia oleracea | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | preferred in an equimolar mixture of palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA | Pisum sativum | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoleoyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoleoyl-CoA + sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
2 isoforms | Pisum sativum |
7.4 | - |
isoforms 1 and 2 | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acyl-CoA | activation, above 0.02 mM, in the presence of bovine serum albumin | Pisum sativum |