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Literature summary for 2.3.1.146 extracted from

  • Kodan, A.; Kuroda, H.; Sakai, F.
    A stilbene synthase from Japanese red pine (Pinus densiflora): implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis (2002), Proc. Natl. Acad. Sci. USA, 99, 3335-3339.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.146

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pinus densiflora

Inhibitors

Inhibitors Comment Organism Structure
pinosylvin potent inhibitor of isozyme PDSTS2 and PDSTS3, noncompetitive Pinus densiflora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 cinnamoyl-CoA isozyme PDSTS3 Pinus densiflora
0.0009
-
 p-Coumaroyl-CoA isozyme PDSTS3 Pinus densiflora
0.0038
-
 cinnamoyl-CoA isozyme PDSTS2 Pinus densiflora
0.0053
-
 p-Coumaroyl-CoA isozyme PDSTS2 Pinus densiflora
0.006
-
 malonyl-CoA isozyme PDSTS2 Pinus densiflora
0.024
-
 malonyl-CoA isozyme PDSTS3 Pinus densiflora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 isozyme PDSTS3, SDS-PAGE Pinus densiflora
42000
-
 isozymes PDSTS1 and PDSTS2, SDS-PAGE Pinus densiflora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamoyl-CoA + malonyl-CoA Pinus densiflora plays a key role in the stilbenoid synthesis 3,5-dihydroxystilbene + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pinus densiflora
-
 Japanese red pine, three isoenzymes PDSTS1, PDSTS2 and PDSTS3
-

Purification (Commentary)

Purification (Comment) Organism
of recombinant enzymes Pinus densiflora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamoyl-CoA + malonyl-CoA
-
 Pinus densiflora 3,5-dihydroxystilbene + CoA + CO2
-
 ?
cinnamoyl-CoA + malonyl-CoA plays a key role in the stilbenoid synthesis Pinus densiflora 3,5-dihydroxystilbene + CoA + CO2
-
 ?
p-coumaroyl-CoA + 3 malonyl-CoA
-
 Pinus densiflora resveratrol + 4 CoA + 4 CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 reaction with cinnamoyl-CoA, isozymes PDSTS2 and PDSTS3 Pinus densiflora
8
-
 reaction with p-coumaroyl-CoA, isozymes PDSTS2 and PDSTS3 Pinus densiflora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.013
-
 pinosylvin isozyme PDSTS2 Pinus densiflora
0.152
-
 pinosylvin isozyme PDSTS3 Pinus densiflora