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Literature summary for 2.3.1.144 extracted from

  • Yang, Q.; Reinhard, K.; Schiltz, E.; Matern, U.
    Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell cultures of carnation, Dianthus caryophyllus L (1997), Plant Mol. Biol., 35, 777-789.
    View publication on PubMed
Search for more literature for 2.3.1.144

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Dianthus caryophyllus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 benzoyl-CoA
-
 Dianthus caryophyllus
0.02
-
 anthranilate
-
 Dianthus caryophyllus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Dianthus caryophyllus
53000
-
 1 * 53000, SDS-PAGE Dianthus caryophyllus

Organism

Organism UniProt Comment Textmining
Dianthus caryophyllus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Q-Sepharose, Blue Sepharose, Sephacryl S200, Mono Q Dianthus caryophyllus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000948
-
-
 Dianthus caryophyllus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + anthranilate 127% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-4-(coumaroyl)anthranilate
-
 ?
benzoyl-CoA + anthranilate cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors Dianthus caryophyllus CoA + N-benzoylanthranilate
-
 ?
cinnamoyl-CoA + anthranilate 140% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-cinnamoylanthranilate
-
 ?
salicyl-CoA + anthranilate 60% of activity with benzoyl-CoA Dianthus caryophyllus CoA + N-salicylanthranilate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Dianthus caryophyllus

Synonyms

Synonyms Comment Organism
anthranilate N-hydroxycinammoyl/benzoyltransferase
-
 Dianthus caryophyllus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7 activity is completely lost if the pH drops below pH 6.5 Dianthus caryophyllus