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Literature summary for 2.3.1.143 extracted from
- Biosynthesis of gallotannins: beta-glucogallin-dependent formation of 1,2,3,4,6-pentagalloylglucose by enzymatic galloylation of 1,2,3,6-tetragalloylglucose (1989), Arch. Biochem. Biophys., 273, 58-63.
General Stability
| General Stability | Organism |
|---|---|
| 0-4°C, within 1-2 weeks no significant loss of activity | Quercus rubra |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
1,2,3,6-tetra-O-galloyl-beta-D-glucose | - |
Quercus rubra |  |
| 2.3 | - |
1-O-Galloyl-beta-D-glucose | - |
Quercus rubra | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
gel filtration | Quercus rubra |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Quercus rubra | - |
oak | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| O2-sensitive, buffers have to be supplemented with 5 mM 2-mercaptoethanol | Quercus rubra |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Quercus rubra |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-O-galloyl-beta-D-glucose + 1,2,3,6-tetrakis-O-galloyl-beta-D-glucose = D-glucose + 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose | stoichiometry | Quercus rubra |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Quercus rubra | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-galloyl-beta-D-glucose + 1,2,3,6-tetra-O-galloyl-beta-D-glucose | 1-O-galloyl-beta-D-glucose is beta-glucogallin, no activity with 1,2,4,6-isomer | Quercus rubra | D-glucose + 1,2,3,4,6-penta-O-galloyl-beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Quercus rubra |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
some activity at 0°C | Quercus rubra |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Quercus rubra |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 50% of maximal activity at pH 4.0 and pH 8.0 | Quercus rubra |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | - |
Quercus rubra |
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