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Literature summary for 2.3.1.142 extracted from

  • Kasinathan, C.; Grzelinska, E.; Okazaki, K.; Slomiany, B.L.; Slomiany, A.
    Purification of protein fatty acyltransferase and determination of its distribution and topology (1990), J. Biol. Chem., 265, 5139-5144.
    View publication on PubMed
Search for more literature for 2.3.1.142

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Rattus norvegicus
Triton X-100 activation Rattus norvegicus

General Stability

General Stability Organism
glycerol, 25% v/v, stabilizes Rattus norvegicus
protease inhibitors stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Rattus norvegicus
MgCl2
-
 Rattus norvegicus
MnCl2
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome rough microsomes, integral part of rough endoplasmic reticulum, exposed to cytoplasmic side Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaF activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus
67000
-
 x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus
243000
-
 native PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + mucus glycoprotein Rattus norvegicus involved in modification of membrane glycoproteins CoA + O-palmitoylglycoprotein
-
 ?
palmitoyl-CoA + mucus glycoprotein Rattus norvegicus Sprague-Dawley involved in modification of membrane glycoproteins CoA + O-palmitoylglycoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
gastric mucosa, solubilized with Triton X-100, affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
gastric mucosa
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
lymphoblast
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
salivary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000934
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + mucus glycoprotein
-
 Rattus norvegicus CoA + O-palmitoylglycoprotein
-
 ?
palmitoyl-CoA + mucus glycoprotein involved in modification of membrane glycoproteins Rattus norvegicus CoA + O-palmitoylglycoprotein
-
 ?
palmitoyl-CoA + mucus glycoprotein
-
 Rattus norvegicus Sprague-Dawley CoA + O-palmitoylglycoprotein
-
 ?
palmitoyl-CoA + mucus glycoprotein involved in modification of membrane glycoproteins Rattus norvegicus Sprague-Dawley CoA + O-palmitoylglycoprotein
-
 ?

Subunits

Subunits Comment Organism
? x * 65000 + x * 67000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus