BRENDA - Enzyme Database show
show all sequences of 2.3.1.142

Purification of protein fatty acyltransferase and determination of its distribution and topology

Kasinathan, C.; Grzelinska, E.; Okazaki, K.; Slomiany, B.L.; Slomiany, A.; J. Biol. Chem. 265, 5139-5144 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activation
Rattus norvegicus
Triton X-100
activation
Rattus norvegicus
General Stability
General Stability
Organism
glycerol, 25% v/v, stabilizes
Rattus norvegicus
protease inhibitors stabilize
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Rattus norvegicus
MgCl2
-
Rattus norvegicus
MnCl2
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
rough microsomes, integral part of rough endoplasmic reticulum, exposed to cytoplasmic side
Rattus norvegicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaF
activation
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
67000
-
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
243000
-
native PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
palmitoyl-CoA + mucus glycoprotein
Rattus norvegicus
involved in modification of membrane glycoproteins
CoA + O-palmitoylglycoprotein
-
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
Rattus norvegicus Sprague-Dawley
involved in modification of membrane glycoproteins
CoA + O-palmitoylglycoprotein
-
Rattus norvegicus Sprague-Dawley
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
Commentary
Organism
gastric mucosa, solubilized with Triton X-100, affinity chromatography
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
gastric mucosa
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
lymphoblast
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
salivary gland
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000934
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-80°C, stable
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
palmitoyl-CoA + mucus glycoprotein
-
486323
Rattus norvegicus
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
involved in modification of membrane glycoproteins
486323
Rattus norvegicus
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
?
palmitoyl-CoA + mucus glycoprotein
involved in modification of membrane glycoproteins
486323
Rattus norvegicus Sprague-Dawley
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
?
Subunits
Subunits
Commentary
Organism
?
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activation
Rattus norvegicus
Triton X-100
activation
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
glycerol, 25% v/v, stabilizes
Rattus norvegicus
protease inhibitors stabilize
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Rattus norvegicus
MgCl2
-
Rattus norvegicus
MnCl2
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
rough microsomes, integral part of rough endoplasmic reticulum, exposed to cytoplasmic side
Rattus norvegicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaF
activation
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
67000
-
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
243000
-
native PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
palmitoyl-CoA + mucus glycoprotein
Rattus norvegicus
involved in modification of membrane glycoproteins
CoA + O-palmitoylglycoprotein
-
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
Rattus norvegicus Sprague-Dawley
involved in modification of membrane glycoproteins
CoA + O-palmitoylglycoprotein
-
Rattus norvegicus Sprague-Dawley
?
Purification (Commentary) (protein specific)
Commentary
Organism
gastric mucosa, solubilized with Triton X-100, affinity chromatography
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
gastric mucosa
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
lymphoblast
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
salivary gland
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000934
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, stable
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
palmitoyl-CoA + mucus glycoprotein
-
486323
Rattus norvegicus
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
involved in modification of membrane glycoproteins
486323
Rattus norvegicus
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus
?
palmitoyl-CoA + mucus glycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
?
palmitoyl-CoA + mucus glycoprotein
involved in modification of membrane glycoproteins
486323
Rattus norvegicus Sprague-Dawley
CoA + O-palmitoylglycoprotein
-
486323
Rattus norvegicus Sprague-Dawley
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 65000 + x * 67000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 2.3.1.142
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720041
Mitchell
The erf4 subunit of the yeast ...
Saccharomyces cerevisiae
J. Biol. Chem.
287
34337-34348
2012
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1
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1
1
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1
-
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1
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1
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1
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-
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2
-
1
-
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1
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-
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1
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1
1
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1
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1
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1
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-
-
2
-
1
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1
-
-
-
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3
3
-
-
-
719950
Zou
Acyl-CoA:lysophosphatidylcholi ...
Mus musculus
J. Biol. Chem.
286
28019-28025
2011
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1
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1
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1
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2
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1
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1
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1
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2
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1
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1
1
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720381
Baghel
Characterization of protein ac ...
Mycobacterium tuberculosis
Microbiol. Res.
166
662-672
2011
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-
1
-
-
-
1
2
1
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2
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3
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3
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1
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1
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1
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1
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2
1
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2
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3
-
1
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1
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2
2
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720984
Singh
Protein acyltransferase functi ...
Haemonchus contortus
Protein Pept. Lett.
18
507-517
2011
-
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1
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1
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2
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4
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-
-
-
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1
1
-
-
-
658184
Hiol
Characterization and partial p ...
Rattus norvegicus
Biochim. Biophys. Acta
1635
10-19
2003
-
-
-
-
-
1
1
-
1
-
-
1
-
2
-
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1
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-
3
2
1
4
-
1
-
1
-
1
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1
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1
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1
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1
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1
-
3
2
1
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
486326
Micchelli
Rasp, a putative transmembrane ...
Drosophila melanogaster
Development
129
843-851
2002
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1
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486325
Basar
Acylation of lysine 983 is suf ...
Bordetella pertussis
J. Biol. Chem.
276
348-354
2001
-
-
1
-
10
-
-
-
-
-
-
1
-
1
-
-
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-
-
-
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2
-
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1
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-
10
-
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1
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2
-
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-
-
-
-
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-
-
-
-
486323
Kasinathan
Purification of protein fatty ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
265
5139-5144
1990
2
-
-
-
-
2
3
-
1
1
3
2
-
163
-
-
1
-
-
8
1
1
4
1
1
-
-
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-
2
-
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-
2
-
3
-
-
1
1
3
2
-
-
-
1
-
8
1
1
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
486324
Schmidt
Solubilization of protein fatt ...
Homo sapiens
Biochem. Soc. Trans.
17
859-861
1989
1
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3
2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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