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Literature summary for 2.3.1.140 extracted from

  • Landmann, C.; Huecherig, S.; Fink, B.; Hoffmann, T.; Dittlein, D.; Coiner, H.A.; Schwab, W.
    Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.) (2011), Planta, 234, 305-320.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS Lavandula angustifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
4-coumaroyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.021
-
3-(4-hydroxyphenyl)lactate with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.022
-
feruloyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.025
-
cinnamoyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.049
-
3-(4-hydroxyphenyl)lactate with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.05
-
caffeoyl-CoA with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.083
-
4-coumaroyl-CoA with shikimate, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.652
-
3-(4-hydroxyphenyl)lactate with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia
0.672
-
3-(4-hydroxyphenyl)lactate with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 Lavandula angustifolia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Lavandula angustifolia 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50600
-
1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate Lavandula angustifolia
-
CoA + rosmarinate
-
?
additional information Lavandula angustifolia recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity ?
-
?

Organism

Organism UniProt Comment Textmining
Lavandula angustifolia Q0GA14
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography Lavandula angustifolia

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Lavandula angustifolia
-

Storage Stability

Storage Stability Organism
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable Lavandula angustifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
-
Lavandula angustifolia CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
-
?
4-coumaroyl-CoA + shikimate
-
Lavandula angustifolia CoA + 5-O-(4-coumaroyl)shikimate
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
-
Lavandula angustifolia CoA + rosmarinate
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
-
Lavandula angustifolia CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
?
cinnamoyl-CoA + 3-(4-hydroxyphenyl)lactate
-
Lavandula angustifolia CoA + 2-O-cinnamoyl-3-(4-hydroxyphenyl)lactate
-
?
feruloyl-CoA + 3-(4-hydroxyphenyl)lactate
-
Lavandula angustifolia CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate
-
?
additional information recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity Lavandula angustifolia ?
-
?
additional information substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid Lavandula angustifolia ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50600, about, LAAT1, sequence determination Lavandula angustifolia

Synonyms

Synonyms Comment Organism
AAT1
-
Lavandula angustifolia
AT1
-
Lavandula angustifolia
rosmarinic acid synthase
-
Lavandula angustifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Lavandula angustifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Lavandula angustifolia

General Information

General Information Comment Organism
evolution rosmarinic acid synthase is a member of the plant BAHD family, it contains the BAHD family HXXXD motif (amino acids 180-184), with the catalytically active histidine and the DFGWG motif that is thought to be responsible for the steric position of the active site, but it additionally contains 25 additional amino acids in the section following the HXXXD motif, phylogenetic tree of BAHD acyltransferases, overview Lavandula angustifolia