Cloned (Comment) | Organism |
---|---|
RAS gene, intron-free and single-copy gene, DNA and amino acid sequence determination and analysis, heterologous expression of N-terminally His6-tagged RAS in Escherichia coli strain BL21(DE3)pLysS | Melissa officinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-coumaroyl-CoA | severe substrate inhibition of 4-coumaroyl-CoA with 3-(4-hydroxyphenyl)lactate as acceptor and less with 3-(3,4-dihydroxyphenyl)lactate | Melissa officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Melissa officinalis | |
0.0023 | - |
caffeoyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.0032 | - |
4-coumaroyl-CoA | with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.0033 | - |
caffeoyl-CoA | with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.0048 | - |
4-coumaroyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0052 | - |
caffeoyl-CoA | with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0058 | - |
caffeoyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0073 | - |
4-coumaroyl-CoA | with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0094 | - |
4-coumaroyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.0169 | - |
3-(4-hydroxyphenyl)lactate | with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0189 | - |
3-(4-hydroxyphenyl)lactate | with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.074 | - |
3-(4-hydroxyphenyl)lactate | with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.0853 | - |
3-(3,4-Dihydroxyphenyl)lactate | with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.1528 | - |
3-(3,4-Dihydroxyphenyl)lactate | with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme | Melissa officinalis | |
0.1565 | - |
3-(4-hydroxyphenyl)lactate | with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.2126 | - |
3-(3,4-Dihydroxyphenyl)lactate | with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme | Melissa officinalis | |
0.2336 | - |
3-(3,4-Dihydroxyphenyl)lactate | with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme | Melissa officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate | Melissa officinalis | - |
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate | - |
? | |
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate | Melissa officinalis | - |
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate | the precursor for rosmarinic acid synthesis via further hydroxylations, overview | ? | |
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate | Melissa officinalis | - |
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Melissa officinalis | - |
RAS gene | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | RAS activity decreases through the cell cultivation period, it is highest after 3-4 days and completely declined after 11 days, overview. The RAS expression level declines also durring cell cultivation and is almost undetectable after 6-9 days, and absent after 10 days | Melissa officinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate | - |
Melissa officinalis | CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate | - |
? | |
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Melissa officinalis | CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate | - |
? | |
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Melissa officinalis | CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate | the precursor for rosmarinic acid synthesis via further hydroxylations, overview | ? | |
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Melissa officinalis | CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumaroyl-CoA:hydroxyphenyllactic acid hydroxycinnamoyltransferase | - |
Melissa officinalis |
RAS | - |
Melissa officinalis |
rosmarinic acid synthase | - |
Melissa officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Melissa officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
recombinant enzyme | Melissa officinalis |
General Information | Comment | Organism |
---|---|---|
evolution | MoRAS belongs to the BAHD superfamily of acyltransferases, it possesses the two conserved sequence motifs HxxxDG and DFGWG | Melissa officinalis |
metabolism | the enzyme catalyzes a step in the rosmarinic acid biosynthesis, pathway overview | Melissa officinalis |