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Literature summary for 2.3.1.137 extracted from

  • Jogl, G.; Hsiao, Y.S.; Tong, L.
    Structure and function of carnitine acyltransferases (2004), Ann. N. Y. Acad. Sci., 1033, 17-29.
    View publication on PubMed
Search for more literature for 2.3.1.137

Protein Variants

Protein Variants Comment Organism
R518N site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme Bos taurus
S552A site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme Bos taurus
S554X site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-
mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octanoyl-CoA + L-carnitine Bos taurus step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio CoA + L-octanoylcarnitine
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine catalytic mechanism, active site structure, substrate recognition mechanism, amino acid residues 343, 564 and 553 determine the acyl-CoA substrate specificity, catalytic residue is His343 Bos taurus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-CoA + L-carnitine step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio Bos taurus CoA + L-octanoylcarnitine
-
 r
octanoyl-CoA + L-carnitine carnitine binding site structure involving the highly conserved R518, Y452 and T465, CoA binding site n the active site tunnel via His343, fatty acid binding site Bos taurus CoA + L-octanoylcarnitine
-
 r

Synonyms

Synonyms Comment Organism
carnitine octanoyltransferase
-
 Bos taurus
CrOT
-
 Bos taurus
More enzyme belongs to the family of carnitine acyltransferases Bos taurus