Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: retinyl heptanoate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs | Homo sapiens | |
0.055 | - |
all-trans-retinol | pH not specified in the publication, 20°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95237 | - |
- |
Purification (Comment) | Organism |
---|---|
SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability. Detergents such as 0.2% Triton X-100, 0.7% CHAPSO,and 1.2 mM n-dodecyl-beta-D-maltoside and sodium are not effective | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
didecanoylphosphatidylcholine + all-trans-retinol | - |
Homo sapiens | all-trans-retinyl decanoate + 2-decanoylglycerophosphocholine | activity is about 10% of that with diheptanoylphosphatidylcholine | ? | |
diheptanoylphosphatidylcholine + all-trans-retinol | - |
Homo sapiens | all-trans-retinyl heptanoate + 2-heptanoylglycerophosphocholine | - |
? | |
dilauroylphosphatidylcholine + all-trans-retinol | - |
Homo sapiens | all-trans-retinyl laurate + 2-lauroylglycerophosphocholine | - |
? | |
dilauroylphosphatidylcholine + all-trans-retinol | - |
Homo sapiens | all-trans-retinyl laurate + 2-lauroylglycerophosphocholine | activity is about 4% of that with diheptanoylphosphatidylcholine | ? | |
dimyristoylphosphatidylcholine + all-trans-retinol | activity is about 3% of that with diheptanoylphosphatidylcholine | Homo sapiens | all-trans-retinyl myristate + 2-myristoylglycerophosphocholine | - |
? | |
dioctanoylphosphatidylcholine + all-trans-retinol | activity is less than 15% of that with diheptanoylphosphatidylcholine | Homo sapiens | all-trans-retinyl octanoate + 2-octanoylglycerophosphocholine | - |
? | |
dioleoylphosphatidylcholine + all-trans-retinol | - |
Homo sapiens | all-trans-retinyl oleate + 2-oleoylglycerophosphocholine | - |
? | |
dipalmitoylphosphatidylcholine + all-trans-retinol | activity is about 2% of that with diheptanoylphosphatidylcholine | Homo sapiens | all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lecithin:retinol acyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | - |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
15% of maximum activity | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40.4 | - |
all-trans-retinol | pH not specified in the publication, 20°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
80% of maximum activity | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
730 | - |
all-trans-retinol | pH not specified in the publication, 20°C | Homo sapiens |