Cloned (Comment) | Organism |
---|---|
expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K104A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
K133A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
K133A/K134A | site-directed mutagenesis, slightly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
K134A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
K147A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
K180A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
K180R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
K186A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
K186R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
K90A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
K95A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
Y118F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
Y154F | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y167F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
Y64F | site-directed mutagenesis, highly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | Homo sapiens | - |
2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95237 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] = 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | mechanism, Cys161 and Tyr154 are essential for activity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of recombinant mutant enzymes and recombinant truncated wild-type, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + all-trans-retinol | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl acylester | - |
? | |
phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? | |
phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lecithin-retinol acyltransferase | - |
Homo sapiens |
LRAT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
about | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile of recombinant mutant enzymes and recombinant truncated wild-type | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10.4 | - |
inactivation of recombinant truncated wild-type enzyme, reversible by dialysis against a buffer with pH 8.4 | Homo sapiens |