Cloned (Comment) | Organism |
---|---|
gene cbhct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged HST in Escherichia coli strain BL21(DE3)pLysS | Coleus scutellarioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Coleus scutellarioides | |
0.0015 | - |
4-coumaroyl-CoA | pH 7.0, 30°C, with 3-hydroxybenzoate | Coleus scutellarioides | |
0.0031 | - |
4-coumaroyl-CoA | pH 7.0, 30°C, with 2,3-dihydroxybenzoate | Coleus scutellarioides | |
0.0043 | - |
4-coumaroyl-CoA | pH 7.0, 30°C, with shikimate | Coleus scutellarioides | |
0.0051 | - |
caffeoyl-CoA | pH 7.0, 30°C, with 3-hydroxybenzoate | Coleus scutellarioides | |
0.0051 | - |
feruloyl-CoA | pH 7.0, 30°C, with shikimate | Coleus scutellarioides | |
0.0088 | - |
caffeoyl-CoA | pH 7.0, 30°C, with shikimate | Coleus scutellarioides | |
0.0118 | - |
caffeoyl-CoA | pH 7.0, 30°C, with 2,3-dihydroxybenzoate | Coleus scutellarioides | |
0.0185 | - |
4-coumaroyl-CoA | pH 7.0, 30°C, with 3-aminobenzoate | Coleus scutellarioides | |
0.02 | - |
sinapoyl-CoA | pH 7.0, 30°C, with shikimate | Coleus scutellarioides | |
0.022 | - |
4-coumaroyl-CoA | pH 7.0, 30°C, with 3-hydroxyanthranilate | Coleus scutellarioides | |
0.0265 | - |
cinnamoyl-CoA | pH 7.0, 30°C, with shikimate | Coleus scutellarioides | |
0.0538 | - |
caffeoyl-CoA | pH 7.0, 30°C, with 3-hydroxyanthranilate | Coleus scutellarioides | |
0.188 | - |
2,3-Dihydroxybenzoate | pH 7.0, 30°C, with 4-coumaroyl-CoA | Coleus scutellarioides | |
0.332 | - |
shikimate | pH 7.0, 30°C, with 4-coumaroyl-CoA | Coleus scutellarioides | |
0.449 | - |
3-Hydroxyanthranilate | pH 7.0, 30°C, with caffeoyl-CoA | Coleus scutellarioides | |
0.473 | - |
3-Hydroxyanthranilate | pH 7.0, 30°C, with 4-coumaroyl-CoA | Coleus scutellarioides | |
0.699 | - |
2,3-Dihydroxybenzoate | pH 7.0, 30°C, with caffeoyl-CoA | Coleus scutellarioides | |
0.733 | - |
3-hydroxybenzoate | pH 7.0, 30°C, with caffeoyl-CoA | Coleus scutellarioides | |
1.677 | - |
3-hydroxybenzoate | pH 7.0, 30°C, with 4-coumaroyl-CoA | Coleus scutellarioides | |
2.235 | - |
shikimate | pH 7.0, 30°C, with cinnamoyl-CoA | Coleus scutellarioides | |
2.404 | - |
3-aminobenzoate | pH 7.0, 30°C, with 4-coumaroyl-CoA | Coleus scutellarioides | |
6.495 | - |
shikimate | pH 7.0, 30°C, with caffeoyl-CoA | Coleus scutellarioides | |
22.38 | - |
shikimate | pH 7.0, 30°C, with feroyl-CoA | Coleus scutellarioides | |
26.13 | - |
shikimate | pH 7.0, 30°C, with sinapoyl-CoA | Coleus scutellarioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47100 | - |
x * 47100, about, sequence calculation | Coleus scutellarioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + shikimate | Coleus scutellarioides | - |
CoA + 5-O-(4-coumaroyl)shikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coleus scutellarioides | E8ZAP2 | gene cbhct2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged HST from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis | Coleus scutellarioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 2,3-dihydroxybenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
4-coumaroyl-CoA + 3-aminobenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
4-coumaroyl-CoA + 3-hydroxyanthranilate | - |
Coleus scutellarioides | CoA + 3-(4-coumaroyl)oxyanthranilate | - |
? | |
4-coumaroyl-CoA + 3-hydroxybenzoate | - |
Coleus scutellarioides | CoA + 3-(4-coumaroyl)oxybenzoate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Coleus scutellarioides | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | preferred substrate for HST with shikimate as acceptor substrate is 4-coumaroyl-CoA | Coleus scutellarioides | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + 2,3-dihydroxybenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
caffeoyl-CoA + 3-aminobenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
caffeoyl-CoA + 3-hydroxyanthranilate | - |
Coleus scutellarioides | CoA + 3-caffeoyloxyanthranilate | - |
? | |
caffeoyl-CoA + 3-hydroxybenzoate | - |
Coleus scutellarioides | CoA + 3-caffeoyloxybenzoate | - |
? | |
caffeoyl-CoA + shikimate | - |
Coleus scutellarioides | CoA + 5-O-caffeoylshikimate | - |
r | |
cinnamoyl-CoA + 2,3-dihydroxybenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
cinnamoyl-CoA + 3-aminobenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
cinnamoyl-CoA + 3-hydroxyanthranilate | - |
Coleus scutellarioides | CoA + 3-cinnamoyloxyanthranilate | - |
? | |
cinnamoyl-CoA + 3-hydroxybenzoate | - |
Coleus scutellarioides | CoA + 3-cinnamoyloxybenzoate | - |
? | |
cinnamoyl-CoA + shikimate | very low activity | Coleus scutellarioides | CoA + 5-O-cinnamoylshikimate | - |
r | |
feruloyl-CoA + 2,3-dihydroxybenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
feruloyl-CoA + 3-aminobenzoate | - |
Coleus scutellarioides | CoA + ? | - |
? | |
feruloyl-CoA + 3-hydroxyanthranilate | - |
Coleus scutellarioides | CoA + 3-feruloyloxyanthranilate | - |
? | |
feruloyl-CoA + 3-hydroxybenzoate | - |
Coleus scutellarioides | CoA + 3-feruloyloxybenzoate | - |
? | |
feruloyl-CoA + shikimate | - |
Coleus scutellarioides | CoA + 5-O-feruloylshikimate | - |
r | |
additional information | substrate specificity of recombinant HST: no activity with quinate and anthranilate, and tyramine, tryptamine, serotonin, dopamine, DOPA, 2-phenethylamine, phenol, as acceptors | Coleus scutellarioides | ? | - |
? | |
sinapoyl-CoA + 3-hydroxyanthranilate | - |
Coleus scutellarioides | CoA + 3-sinapoyloxyanthranilate | - |
r | |
sinapoyl-CoA + shikimate | very low activity | Coleus scutellarioides | CoA + 5-O-sinapoylshikimate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 47100, about, sequence calculation | Coleus scutellarioides |
Synonyms | Comment | Organism |
---|---|---|
HST | - |
Coleus scutellarioides |
hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase | - |
Coleus scutellarioides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coleus scutellarioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Coleus scutellarioides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Coleus scutellarioides | sequence calculation | - |
5.78 |
General Information | Comment | Organism |
---|---|---|
evolution | HST belongs to the large plant acyl-CoA-dependent BAHD acyltransferase superfamily characterized by mostly conserved sequence motifs, the HxxxD(G) motif containing the catalytically active histidine residue and the DFGWG motif close to the C terminus, phylogenetic analysis, overview | Coleus scutellarioides |