Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trifolium pratense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48300 | - |
calculated from cDNA | Trifolium pratense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trifolium pratense | B6D7P1 | cDNA HCT1A (almost identical to HCT1B) | - |
Trifolium pratense | B6D7P2 | - |
- |
Trifolium pratense | B6DQK4 | cDNA HCT1B (almost identical to HCT1A) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves | Trifolium pratense | - |
leaf | HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems | Trifolium pratense | - |
stem | expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves | Trifolium pratense | - |
stem | HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems | Trifolium pratense | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + L-malate | - |
Trifolium pratense | CoA + 2-O-(4-coumaryl)-L-malate | - |
? | |
4-coumaroyl-CoA + quinate | reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor | Trifolium pratense | CoA + 4-coumaroylquinate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Trifolium pratense | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + L-malate | - |
Trifolium pratense | CoA + 2-O-caffeoyl-L-malate | - |
? | |
caffeoyl-CoA + quinate | reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor | Trifolium pratense | CoA + caffeoylquinate | - |
? | |
caffeoyl-CoA + shikimate | - |
Trifolium pratense | CoA + 5-O-caffeoylshikimate | - |
? | |
additional information | no reaction products are detected when HCT1 and either hydroxycinnamoyl-CoA derivative are incubated with malic acid, Glc, or L-DOPA as acceptor | Trifolium pratense | ? | - |
? | |
additional information | no reaction products are detected when shikimic acid, quinic acid, Glc, or L-DOPA is used as an acceptor | Trifolium pratense | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCT1 | two cDNAs are isolated HCT1A and HCT1B which are nearly identical | Trifolium pratense |
HCT2 | - |
Trifolium pratense |
hydroxycinnamoyltransferase | - |
Trifolium pratense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trifolium pratense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trifolium pratense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 6.7 | optimal pH range with malate as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 6.1 and 6.7 using sodium phosphate buffer | Trifolium pratense |
7.5 | 7.9 | optimal pH range with shikimic acid as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 7.5 and 7.9 using sodium phosphate buffer | Trifolium pratense |