Cloned (Comment) | Organism |
---|---|
- |
Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
E226Q | about 3fold increase in Km value for lgycine | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
glycine | wild-type, pH 8.0, 30°C | Bos taurus | |
0.002 | - |
glycine | recombinant wild-type, pH 8.0, 30°C | Bos taurus | |
0.007 | - |
glycine | mutant E226Q, pH 8.0, 30°C | Bos taurus | |
0.016 | - |
benzoyl-CoA | wild-type, pH 8.0, 30°C | Bos taurus | |
0.016 | - |
benzoyl-CoA | recombinant wild-type, pH 8.0, 30°C | Bos taurus | |
0.018 | - |
benzoyl-CoA | mutant E226Q, pH 8.0, 30°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q2KIR7 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + glycine = CoA + N-acylglycine | residue Glu226 functions to deprotonate glycine, facilitating nucleophilic attack on the acyl-CoA substrate | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + glycine | - |
Bos taurus | CoA + N-benzoylglycine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
for mutant E226Q, activity increases significantly when raising the pH above 8.0, while for wild-type, activity remains more or less stable up to pH 9.0 | Bos taurus |
7.5 | 8.5 | wild-type | Bos taurus |