Inhibitors | Comment | Organism | Structure |
---|---|---|---|
myristoyl-[acyl-carrier protein] | - |
Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the kinetic analysis of LpxA is complicated by the thermodynamically unfavorable reaction in the forward direction, overview | Vibrio harveyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not as effective as Mg2+ | Vibrio harveyi | |
Mg2+ | is required for stabilization of substrate acyl-carrier protein in a helical conformation | Vibrio harveyi | |
Mn2+ | not as effective as Mg2+ | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Vibrio harveyi | - |
[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
gene lpxA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Vibrio harveyi | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | i.e. (R)-3-hydroxymyristoyl-[acyl-carrier protein], the forward reaction direction is thermodynamically unfavorable, recombinant His-tagged enzyme, the activity with mutant acyl-carrier proteins is impaired, especially with mutations of the B-site of the acyl-carrier protein, recombinant wild-type and mutant ACP substrates, overview | Vibrio harveyi | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LpxA | - |
Vibrio harveyi |
UDP-N-acetylglucosamine acyltransferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vibrio harveyi |