Cloned (Comment) | Organism |
---|---|
gene lpxA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Acidithiobacillus ferrooxidans |
gene lpxA, expression in Escherichia coli strain BL21(DE3) | Leptospira interrogans |
gene lpxA, expression in strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | heterologous expression of Acidithiobacillus ferrooxidans' genes lpxA, gnnA, and gnnB results in occurence of UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose utilizing activity in Escherichia coli cells with defective lpxA gene in vivo | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | Leptospira interrogans | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | Mesorhizobium loti | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | Acidithiobacillus ferrooxidans | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Escherichia coli | - |
[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | Q6QHI7 | - |
- |
Escherichia coli | - |
- |
- |
Leptospira interrogans | - |
- |
- |
Mesorhizobium loti | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier protein] + a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine | mechanism involving essential His125 | Leptospira interrogans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of the recombinant enzyme | Acidithiobacillus ferrooxidans |
additional information | - |
substrate specificity with different acyl acceptors | Mesorhizobium loti |
additional information | - |
substrate specificity with different acyl acceptors, activity of the recombinant enzyme | Escherichia coli |
additional information | - |
substrate specificity with different acyl acceptors, activity of the recombinant enzyme | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
Mesorhizobium loti | [acyl-carrier protein] + UDP-3-N-(3-hydroxydecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
Mesorhizobium loti | [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | Leptospira interrogans | [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | the enzyme is absolute specific for 3-hydroxylauroyl-ACP as donor substrate | Leptospira interrogans | [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
(R)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
Mesorhizobium loti | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | Mesorhizobium loti | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units | Acidithiobacillus ferrooxidans | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | only in vitro | Escherichia coli | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | specific for UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | Acidithiobacillus ferrooxidans | [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | low activity | Acidithiobacillus ferrooxidans | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
additional information | substrate specificity, lipid A structure overview | Acidithiobacillus ferrooxidans | ? | - |
? | |
additional information | substrate specificity, no activity with UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose due to lack of genes gnnA and gnnB, lipid A structure overview | Escherichia coli | ? | - |
? | |
additional information | substrate specificity, no activity with UDP-N-acetylglucosamine, equally effective with 3-hydroxyacyl-ACP of different chain lengths, lipid A structure overview | Mesorhizobium loti | ? | - |
? | |
additional information | substrate specificity, no activity with UDP-N-acetylglucosamine, lipid A structure overview | Leptospira interrogans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LpxA | - |
Escherichia coli |
LpxA | - |
Mesorhizobium loti |
LpxA | - |
Leptospira interrogans |
LpxA | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Mesorhizobium loti |
30 | - |
assay at | Leptospira interrogans |
30 | - |
assay at | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Mesorhizobium loti |
8 | - |
assay at | Leptospira interrogans |
8 | - |
assay at | Acidithiobacillus ferrooxidans |