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Literature summary for 2.3.1.129 extracted from

  • Sweet, C.R.; Williams, A.H.; Karbarz, M.J.; Werts, C.; Kalb, S.R.; Cotter, R.J.; Raetz, C.R.
    Enzymatic synthesis of lipid A molecules with four amide-linked acyl chains. LpxA acyltransferases selective for an analog of UDP-N-acetylglucosamine in which an amine replaces the 3"-hydroxyl group (2004), J. Biol. Chem., 279, 25411-25419.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene lpxA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Acidithiobacillus ferrooxidans
gene lpxA, expression in Escherichia coli strain BL21(DE3) Leptospira interrogans
gene lpxA, expression in strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information heterologous expression of Acidithiobacillus ferrooxidans' genes lpxA, gnnA, and gnnB results in occurence of UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose utilizing activity in Escherichia coli cells with defective lpxA gene in vivo Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Leptospira interrogans first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Escherichia coli
-
[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans Q6QHI7
-
-
Escherichia coli
-
-
-
Leptospira interrogans
-
-
-
Mesorhizobium loti
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier protein] + a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine mechanism involving essential His125 Leptospira interrogans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of the recombinant enzyme Acidithiobacillus ferrooxidans
additional information
-
substrate specificity with different acyl acceptors Mesorhizobium loti
additional information
-
substrate specificity with different acyl acceptors, activity of the recombinant enzyme Escherichia coli
additional information
-
substrate specificity with different acyl acceptors, activity of the recombinant enzyme Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxydecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Leptospira interrogans [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose the enzyme is absolute specific for 3-hydroxylauroyl-ACP as donor substrate Leptospira interrogans [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine
-
?
(R)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose only in vitro Escherichia coli [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose specific for UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine low activity Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
?
additional information substrate specificity, lipid A structure overview Acidithiobacillus ferrooxidans ?
-
?
additional information substrate specificity, no activity with UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose due to lack of genes gnnA and gnnB, lipid A structure overview Escherichia coli ?
-
?
additional information substrate specificity, no activity with UDP-N-acetylglucosamine, equally effective with 3-hydroxyacyl-ACP of different chain lengths, lipid A structure overview Mesorhizobium loti ?
-
?
additional information substrate specificity, no activity with UDP-N-acetylglucosamine, lipid A structure overview Leptospira interrogans ?
-
?

Synonyms

Synonyms Comment Organism
LpxA
-
Escherichia coli
LpxA
-
Mesorhizobium loti
LpxA
-
Leptospira interrogans
LpxA
-
Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli
30
-
assay at Mesorhizobium loti
30
-
assay at Leptospira interrogans
30
-
assay at Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli
8
-
assay at Mesorhizobium loti
8
-
assay at Leptospira interrogans
8
-
assay at Acidithiobacillus ferrooxidans