Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.129 extracted from

  • Anderson, M.S.; Bull, H.G.; Galloway, S.M.; Kelly, T.M.; Mohan, S.; Radika, K.; Raetz, C.R.
    UDP-N-acetylglucosamine acyltransferase of Escherichia coli. The first step of endotoxin biosynthesis is thermodynamically unfavorable (1993), J. Biol. Chem., 268, 19858-19865.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin prevents denaturation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
KCl 0.5 M Escherichia coli
LiCl 0.5 M Escherichia coli
additional information no inhibition by N-ethylmaleimide, UDP-2,3-diacyl-N-glucosamine, lipid X, lipid IVA, 3-deoxy-D-mannooctulosonate, octyl-beta-D-glucoside Escherichia coli
myristoyl-[acyl-carrier protein]
-
Escherichia coli
NaCl 0.5 M Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein]
-
Escherichia coli
0.099
-
UDP-N-acetylglucosamine
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl slight activation at 0.25 M Escherichia coli
LiCl slight activation at 0.25 M Escherichia coli
NaCl slight activation at 0.25 M Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, calculated from nucleotide sequence Escherichia coli
30000
-
alpha2, 2 * 30000, SDS-PAGE Escherichia coli
30000
-
alpha3, 3 * 30000, SDS-PAGE Escherichia coli
90000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
MC1061/pSR1 (overproducing strain harbouring plasmid pSR1)
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.3
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein] Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
r
(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine high specificity with respect to acyl-donor, equilibrium constant favors thioester acyl carrier substrate Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
r

Subunits

Subunits Comment Organism
? x * 27000, calculated from nucleotide sequence Escherichia coli
dimer or trimer alpha2, 2 * 30000, SDS-PAGE Escherichia coli
trimer alpha3, 3 * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8.2 broad Escherichia coli