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Literature summary for 2.3.1.129 extracted from
- UDP-N-acetylglucosamine 3-O-acyltransferase from Escherichia coli (1992), Methods Enzymol., 209, 449-454.
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing inactivates | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by N-ethylmaleimide, UDP-2,3-diacyl-N-glucosamine, lipid X, lipid IVA, 3-deoxy-D-mannooctulosonate, octyl-beta-D-glucoside | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, calculated from nucleotide sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Escherichia coli | essential for cell growth of gram-negative bacteria | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? |  |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Escherichia coli | involved with EC 2.4.1.182 and 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid and outer membrane component, Lipid A | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Escherichia coli | initial reaction in lipid A biosynthesis | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
MC1061/pSR1 (overproducing strain harbouring plasmid pSR1) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, overproducing strain MC1061/pSR1 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.92 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, flash-frozen in liquid nitrogen, at least 3 months | Escherichia coli |
| 4°C, several days | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | essential for cell growth of gram-negative bacteria | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | involved with EC 2.4.1.182 and 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid and outer membrane component, Lipid A | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | initial reaction in lipid A biosynthesis | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-butyrylglucosamine | transacylation at 8% the rate of the reaction with UDP-N-acetylglucosamine | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-butyrylglucosamine | - |
? | |
| (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-propionyl-alpha-D-glucosamine | transacylation at 22% the rate of the reaction with UDP-N-acetylglucosamine | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-propionyl-alpha-D-glucosamine | - |
? | |
| (R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | i.e. 3-hydroxylauroyl-[acyl-carrier-protein], poor substrate | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein] | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
| (S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | transacylation at 7% the rate of (R)-enantiomer | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, calculated from nucleotide sequence | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
20 min stable | Escherichia coli |
| 100 | - |
inactivation after 10 min | Escherichia coli |
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Release 2023.1 (January 2023)
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