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Literature summary for 2.3.1.126 extracted from
- Hydroxycinnamic acid esters of isocitric acid: Accumulation and enzymatic synthesis in Amaranthus cruentus (1987), Phytochemistry, 26, 2919-2923.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
caffeoyl-CoA | - |
Amaranthus cruentus |  |
| 0.055 | - |
4-coumaroyl-CoA | - |
Amaranthus cruentus | |
| 0.163 | - |
feruloyl-CoA | - |
Amaranthus cruentus | |
| 0.208 | - |
isocitric acid | second substrate caffeoyl-CoA | Amaranthus cruentus | |
| 0.257 | - |
isocitric acid | second substrate p-coumaroyl-CoA | Amaranthus cruentus | |
| 0.412 | - |
isocitric acid | second substrate feruloyl-CoA | Amaranthus cruentus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + isocitrate | Amaranthus hybridus | - |
CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | Amaranthus tricolor | - |
CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | Amaranthus cruentus | - |
CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | Amaranthus paniculatus | - |
CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | Amaranthus paniculatus L. | - |
CoA + 2-caffeoylisocitrate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amaranthus cruentus | - |
Willd. | - |
| Amaranthus cruentus | - |
L. cv. Oeschberg | - |
| Amaranthus hybridus | - |
ssp. paniculatus (L.) Hejny | - |
| Amaranthus paniculatus | - |
L. | - |
| Amaranthus paniculatus L. | - |
L. | - |
| Amaranthus tricolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Amaranthus cruentus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Amaranthus cruentus | - |
| leaf | - |
Amaranthus cruentus | - |
| seedling | - |
Amaranthus cruentus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protein preparation shows no loss of enzymatic activity when stored for several weeks | Amaranthus cruentus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + isocitrate | - |
Amaranthus cruentus | CoA + 4-coumaroylisocitrate | - |
? | |
| caffeoyl-CoA + isocitrate | - |
Amaranthus hybridus | CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | - |
Amaranthus tricolor | CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | - |
Amaranthus cruentus | CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | - |
Amaranthus paniculatus | CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitrate | - |
Amaranthus paniculatus L. | CoA + 2-caffeoylisocitrate | - |
r | |
| caffeoyl-CoA + isocitric acid | - |
Amaranthus cruentus | CoA + (E)-caffeoylisocitric acid | - |
r | |
| feruloyl-CoA + isocitric acid | - |
Amaranthus cruentus | CoA + feruloylisocitric acid | - |
r | |
| additional information | citric, malic tartaric and quinic acids are not accepted as substrates | Amaranthus cruentus | ? | - |
? | |
| sinapoyl-CoA + isocitric acid | minor activity compared to caffeoyl-CoA | Amaranthus cruentus | CoA + sinapoylisocitric acid | - |
r | |
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