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Literature summary for 2.3.1.12 extracted from
- Structure and function of the catalytic domain of the dihydrolipoyl acetyltransferase component in Escherichia coli pyruvate dehydrogenase complex (2014), J. Biol. Chem., 289, 15215-15230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| His9-tagged catalytic domain, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) polyethylene glycol 3350 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H602C | inactive | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30028 | - |
x * 30028, catalytic domain, calculated from amino acid sequence | Escherichia coli |
| 30030 | - |
x * 30030, catalytic domain, FT mass spectrometry | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | Escherichia coli | - |
CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P06959 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity resin column chromatography, Superdex G75 gel filtration, and G3000SW TSK gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
Escherichia coli | CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
r | |
| CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
Escherichia coli | acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30028, catalytic domain, calculated from amino acid sequence | Escherichia coli |
| ? | x * 30030, catalytic domain, FT mass spectrometry | Escherichia coli |
| trimer | x-ray crystallography | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide acetyltransferase | - |
Escherichia coli |
| pyruvate dehydrogenase complex component E2p | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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