Cloned (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
G610C | 500fold reduced enzymatic activity | Azotobacter vinelandii |
N614D | 9fold reduced enzymatic activity | Azotobacter vinelandii |
S558A | 200fold reduced enzymatic activity | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
acetyl-CoA | wild-type enzyme | Azotobacter vinelandii | |
0.028 | - |
acetyl-CoA | S558A mutant | Azotobacter vinelandii | |
0.036 | - |
acetyl-CoA | H610C mutant | Azotobacter vinelandii | |
0.052 | - |
acetyl-CoA | N614D mutant | Azotobacter vinelandii | |
1.08 | - |
dihydrolipoamide | N614D mutant | Azotobacter vinelandii | |
5.3 | - |
dihydrolipoamide | H610C mutant | Azotobacter vinelandii | |
5.37 | - |
dihydrolipoamide | wild-type enzyme | Azotobacter vinelandii | |
6.4 | - |
dihydrolipoamide | S558A mutant | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + acetyl-CoA | Azotobacter vinelandii | - |
S-acetyldihydrolipoamide + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
catalytic domain mutants | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + acetyl-CoA | - |
Azotobacter vinelandii | S-acetyldihydrolipoamide + CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00417 | - |
acetyl-CoA | with dihydrolipoamide, H610C mutant | Azotobacter vinelandii | |
0.0143 | - |
acetyl-CoA | with dihydrolipoamide, S558A mutant | Azotobacter vinelandii | |
0.282 | - |
acetyl-CoA | with dihydrolipoamide, N614D mutant | Azotobacter vinelandii | |
2.4 | - |
acetyl-CoA | with dihydrolipoamide, wild-type enzyme | Azotobacter vinelandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.4 | stable | Azotobacter vinelandii |