Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterobacter sp. CZ-1 |
Protein Variants | Comment | Organism |
---|---|---|
C69A | mutation in isoform NhoA1, inactive | Enterobacter sp. CZ-1 |
C72A | mutation in isoform NhoA2, inactive | Enterobacter sp. CZ-1 |
R65A | mutation if isoform NhoA1, inactive | Enterobacter sp. CZ-1 |
R68A | mutation in isoform NhoA2, inactive | Enterobacter sp. CZ-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | moderate inhibition of isoform NhoA1 | Enterobacter sp. CZ-1 | |
Cr3+ | moderate inhibition of isoform NhoA1 | Enterobacter sp. CZ-1 | |
Fe3+ | 1 mM, complete inhibition of isoform NhoA1 | Enterobacter sp. CZ-1 | |
N-ethylmaleimide | 1 mM, 65% inhibition of isoform NhoA1, 45% inhibition of isoform NhoA2 | Enterobacter sp. CZ-1 | |
paraoxon | 0.1 mM, 23.5% inhibition of isoform NhoA1, 14% inhibition of isoform NhoA2 | Enterobacter sp. CZ-1 | |
Zn2+ | moderate inhibition of both isoforms NhoA1 and NhoA2 | Enterobacter sp. CZ-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.151 | - |
3-amino-4-hydroxyphenylarsonic acid | isoform NhoA1, pH 7.4, 30°C | Enterobacter sp. CZ-1 | |
0.428 | - |
3-amino-4-hydroxyphenylarsonic acid | isoform NhoA2, pH 7.4, 30°C | Enterobacter sp. CZ-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
gel filtration, isoform NhoA2 | Enterobacter sp. CZ-1 |
66000 | - |
gel filtration, isoform NhoA1 | Enterobacter sp. CZ-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. CZ-1 | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, elution buffer, 1 month, 89% residual activity for isoform NhoA1, 95% residual activity for isoform NhoA2, respectively | Enterobacter sp. CZ-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-amino-4-hydroxyphenylarsonic acid | - |
Enterobacter sp. CZ-1 | CoA + N-acetyl-3-amino-4-hydroxyphenylarsonic acid | - |
? | |
additional information | no substrates of either isoform NhoA1 or NhoA2: 4-amino-phenylarsonic acid, 2-amino-phenylarsonic acid, and 4-carbamoylamino-phenylarsonic acid | Enterobacter sp. CZ-1 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29600, SDS-PAGE, isoform NhoA2 | Enterobacter sp. CZ-1 |
dimer | 2 * 32700, SDS-PAGE, isoform NhoA1 | Enterobacter sp. CZ-1 |
Synonyms | Comment | Organism |
---|---|---|
NhoA1 | - |
Enterobacter sp. CZ-1 |
NhoA2 | - |
Enterobacter sp. CZ-1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.34 | - |
3-amino-4-hydroxyphenylarsonic acid | isoform NhoA2, pH 7.4, 30°C | Enterobacter sp. CZ-1 | |
54.5 | - |
3-amino-4-hydroxyphenylarsonic acid | isoform NhoA1, pH 7.4, 30°C | Enterobacter sp. CZ-1 |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion of of isoforms NhoA1 and NhoA2 decreases 3-amino-4-hydroxyphenylarsonic acid acetylation activity by 97 and 14%, respectively, the double mutant loses the acetylation activity completely. Deletion of isoform NhoA1 increases the release of inorganic As(III) and As(V) from3 -amino-4-hydroxyphenylarsonic acid or roxarsone | Enterobacter sp. CZ-1 |