Literature summary for 2.3.1.115 extracted from

Ahmad, M.Z.; Li, P.; Wang, J.; Rehman, N.U.; Zhao, J.
Isoflavone malonyltransferases GmIMaT1 and GmIMaT3 differently modify isoflavone glucosides in soybean (Glycine max) under various stresses (2017), Front. Plant Sci., 8, 735 .

Search for more literature for 2.3.1.115

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0129	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max
0.0131	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max
0.0183	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
0.023	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
0.0256	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
0.0267	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
0.0301	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max
0.0363	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Glycine max	5829	-
endoplasmic reticulum	-	Glycine max	5783	-

Organism

Organism	UniProt	Comment	Textmining
Glycine max	A0A1X9JQM7	-	-
Glycine max	A0A1Y9TFD9	isoform IMaT3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonyl-CoA + daidzein 7-O-glucoside	-	Glycine max	CoA + daidzein 7-O-glucoside 6''-O-malonylester	-	?
malonyl-CoA + genistein 7-O-glucoside	-	Glycine max	CoA + genistein 7-O-glucoside 6''-O-malonylester	-	?
malonyl-CoA + glycitein 7-O-glucoside	-	Glycine max	CoA + glycitein 7-O-glucoside 6''-O-malonylester	-	?
additional information	IMaT1 prefers to use malonyl-CoA, rather than acetyl CoA, as an acyl donor to convert only isoflavone 7-O-glucosides, but not other flavonoid glucosides	Glycine max	?	-	-

Synonyms

Synonyms	Comment	Organism
IMaT1	-	Glycine max
IMaT3	-	Glycine max
isoflavone malonyltransferase	-	Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.1	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
19.21	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
29.6	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max
34.24	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max
40.95	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
56.4	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
66.65	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max
68.4	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	substrates malonyl-CoA and genistin	Glycine max

General Information

General Information	Comment	Organism
physiological function	transgenic hairy roots overexpressing IMaT1 have increased levels of malonyldaidzin and malonylgenistin. Knockdown of IMaT1 reduces malonyldaidzin and malonylgenistin contents, and affects other isoflavonoids differently	Glycine max
physiological function	transgenic hairy roots overexpressing IMaT3 have increased levels of malonyldaidzin and malonylgenistin. Knockdown of IMaT3 reduces malonyldaidzin and malonylgenistin contents, and affects other isoflavonoids differently	Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
440	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
750	-	malonyl-CoA	cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C	Glycine max
820	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max
1150	-	daidzein 7-O-glucoside	pH 7.2, 30°C	Glycine max
1540	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
2470	-	genistein 7-O-glucoside	pH 7.2, 30°C	Glycine max
5210	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max
5610	-	glycitein 7-O-glucoside	pH 7.2, 30°C	Glycine max

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Release 2023.1 (January 2023)