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Literature summary for 2.3.1.115 extracted from

  • Koester, J.; Bussmann, R.; Barz, W.
    Malonyl-coenzyme A:isoflavone 7-O-glucoside-6-O-malonyltransferase from roots of chick pea (Cicer arietinum L.) (1984), Arch. Biochem. Biophys., 234, 513-521.
    View publication on PubMed
Search for more literature for 2.3.1.115

General Stability

General Stability Organism
most stable in Tris buffer at pH 8, unstable to repeated freezing and thawing, dithioerythritol stabilizes Cicer arietinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 formononetin 7-O-glucoside
-
 Cicer arietinum
0.036
-
 Biochanin A-beta-D-glucoside
-
 Cicer arietinum
0.048
-
 malonyl-CoA
-
 Cicer arietinum
0.054
-
 (-)-maackiain 3-O-beta-D-glucoside
-
 Cicer arietinum
0.065
-
 daidzein 7-O-glucoside
-
 Cicer arietinum
0.067
-
 kaempferol 7-O-glucoside
-
 Cicer arietinum
0.088
-
 genistein 7-O-glucoside
-
 Cicer arietinum
0.098
-
 pratensein 7-O-glucoside
-
 Cicer arietinum
0.101
-
 succinyl-CoA
-
 Cicer arietinum
0.11
-
 2',4,4'-trihydroxychalcone 4'-O-glucoside
-
 Cicer arietinum
0.16
-
 apigenin 7-O-glucoside
-
 Cicer arietinum
0.335
-
 quercetagetin 7-O-glucoside
-
 Cicer arietinum
0.381
-
 quercetin 7-O-glucoside
-
 Cicer arietinum
0.46
-
 orobol 7-O-glucoside
-
 Cicer arietinum
0.47
-
 luteolin 7-O-glucoside
-
 Cicer arietinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
apigenin 7-O-apiosylglucoside + malonyl-CoA Cicer arietinum i.e. apiin apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
 chick pea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cicer arietinum

Source Tissue

Source Tissue Comment Organism Textmining
root 4 days old Cicer arietinum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.515
-
-
 Cicer arietinum

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, stable for 4 months Cicer arietinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin 7-O-apiosylglucoside + malonyl-CoA i.e. apiin Cicer arietinum apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
 ?
apigenin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
 ?
luteolin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
 ?
malonyl CoA + genistein 7-O-glucoside
-
 Cicer arietinum CoA + genistein 7-O-glucoside 6''-O-malonylester
-
 ?
malonyl-CoA + (-)-maackiain 3-O-beta-D-glucoside
-
 Cicer arietinum CoA + (-)-maackiain 3-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside
-
 Cicer arietinum CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside 6''-O-malonylester
-
 ?
malonyl-CoA + biochanin A 7-O-glucoside
-
 Cicer arietinum CoA + biochanin A 7-O-glucoside-6''-O-malonylester
-
 ?
malonyl-CoA + biochanin A-beta-D-glucoside
-
 Cicer arietinum coenzyme A + biochanin A 7-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
malonyl-CoA + daidzein 7-O-glucoside
-
 Cicer arietinum CoA + daidzein 7-O-glucoside 6''-O-malonylester
-
 ?
malonyl-CoA + formononetin 7-O-glucoside
-
 Cicer arietinum CoA + formononetin 7-O-glucoside-6''-O-malonylester
-
 ?
malonyl-CoA + kaempferol 7-O-glucoside
-
 Cicer arietinum CoA + kaempferol 7-O-glucoside 6''-O-malonylester
-
 ?
malonyl-CoA + orobol 7-O-glucoside
-
 Cicer arietinum CoA + orobol 7-O-glucoside 6''-O-malonylester
-
 ?
malonyl-CoA + pratensein 7-O-glucoside
-
 Cicer arietinum CoA + pratensein 7-O-glucoside 6''-O-malonylester
-
 ?
additional information isoflavones with the glucosyl substituent in position 4' don't act as acceptors Cicer arietinum ?
-
 ?
additional information succinyl-CoA can replace malonyl-CoA with lower activity Cicer arietinum ?
-
 ?
quercetagetin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + quercetagetin 7-O-glucoside 6''-O-malonylester
-
 ?
quercetin 7-O-glucoside + malonyl-CoA
-
 Cicer arietinum CoA + quercetin 7-O-glucoside 6''-O-malonylester
-
 ?
succinyl-CoA + biochanin A-beta-D-glucoside
-
 Cicer arietinum coenzyme A + biochanin A 7-O-(6-O-succinyl-beta-D-glucoside)
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Cicer arietinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 above 2 min, destroyed Cicer arietinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cicer arietinum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 about 50% of activity maximum at pH 6.5 and 9.5 Cicer arietinum