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Literature summary for 2.3.1.114 extracted from

  • Sandermann, Jr.H.; Schmitt, r.; Eckey, H.; Bauknecht, T.
    Plant biochemistry of xenobiotics: isolation and properties of soybean O- and N-glucosyl and O- and N-malonyltransferases for chlorinated phenols and anilines (1991), Arch. Biochem. Biophys., 287, 341-350.
    View publication on PubMed
Search for more literature for 2.3.1.114

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, complete inhibition, activity can be restored by incubation with 2 mM dithiothreitol for 30 min at 0°C Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 3,4-dichloroaniline
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 1 * 47000, SDS-PAGE Glycine max
48000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, ion-exchange, Sephadex G-100, hydrophobic interaction, gel filtration Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.143
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 2,3,4,5-tetrachloroaniline 29% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2,3,4,5-tetrachlorophenyl)-malonamate
-
 ?
malonyl-CoA + 2,3,4-trichloroaniline 50% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2,3,4-trichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 2,4,5-trichloroaniline 22% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2,4,5-trichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 2,4-dichloroaniline 53% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2,4-dichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 2,5-dichloroaniline 53% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2,5-dichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 2-chloroaniline 61% of activity with 3,4-dichloroaniline Glycine max CoA + N-(2-chlorophenyl)-malonamate
-
 ?
malonyl-CoA + 3,4,5-trichloroaniline 72% of activity with 3,4-dichloroaniline Glycine max CoA + N-(3,4,5-trichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 3,4,5-trichloroaniline 30% of activity with 3,4-dichloroaniline Glycine max CoA + N-(3,4,5-trichloro-phenyl)-malonamate
-
 ?
malonyl-CoA + 3,4-dichloroaniline preferred substrate Glycine max CoA + N-(3,4-dichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 3,5-dichloroaniline 48% of activity with 3,4-dichloroaniline Glycine max CoA + N-(3,5-dichlorophenyl)-malonamate
-
 ?
malonyl-CoA + 3-chloroaniline 67% of activity with 3,4-dichloroaniline Glycine max CoA + N-(3-chlorophenyl)-malonamate
-
 ?
malonyl-CoA + 4-aminophenol 20% of activity with 3,4-dichloroaniline Glycine max CoA + N-(phenyl)-malonamate
-
 ?
malonyl-CoA + 4-bromoaniline 75% of activity with 3,4-dichloroaniline Glycine max CoA + N-(4-bromophenyl)-malonamate
-
 ?
malonyl-CoA + 4-chloroaniline 87% of activity with 3,4-dichloroaniline Glycine max CoA + N-(4-chlorophenyl)-malonamate
-
 ?
malonyl-CoA + 4-nitroaniline 43% of activity with 3,4-dichloroaniline Glycine max CoA + N-(4-nitrophenyl)-malonamate
-
 ?
malonyl-CoA + 7-amino-4-methylcoumarin 70% of activity with 3,4-dichloroaniline Glycine max N-(4-methylcoumaryl)-7-malonamate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 7.2
-
 Glycine max