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Literature summary for 2.3.1.110 extracted from
- Purification, characterization, and immunolocalization of hydroxycinnamoyl-CoA: tyramine N-(hydroxycinnamoyl)transferase from opium poppy (1999), Planta, 209, 33-44.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | almost complete inactivation at 1 mM | Papaver somniferum |  |
| FeSO4 | almost complete inactivation at 1 mM | Papaver somniferum | |
| ZnCl2 | almost complete inactivation at 1 mM | Papaver somniferum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
cinnamoyl-CoA | - |
Papaver somniferum | |
| 0.017 | - |
4-coumaroyl-CoA | - |
Papaver somniferum | |
| 0.021 | - |
sinapoyl-CoA | - |
Papaver somniferum | |
| 0.062 | - |
feruloyl-CoA | - |
Papaver somniferum | |
| 0.076 | - |
tyramine | - |
Papaver somniferum | |
| 0.078 | - |
dopamine | - |
Papaver somniferum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
2 * 25000, SDS-PAGE | Papaver somniferum |
| 50000 | - |
gel filtration | Papaver somniferum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl-CoA + tyramine | Papaver somniferum | - |
CoA + feruloyltyramine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papaver somniferum | - |
opium poppy | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 988fold | Papaver somniferum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anther | - |
Papaver somniferum | - |
| root | xylem | Papaver somniferum | - |
| seed | - |
Papaver somniferum | - |
| xylem | - |
Papaver somniferum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.76 | - |
- |
Papaver somniferum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, no loss of activity for 2 months in the presence of 2-mercaptoethanol | Papaver somniferum |
| -80°C, no loss of activity for 4 months in the presence of 2-mercaptoethanol | Papaver somniferum |
| 4°C, no loss of activity for 14 days in the presence of 2-mercaptoethanol | Papaver somniferum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + tyramine | - |
Papaver somniferum | CoA + N-(4-coumaroyl)tyramine | - |
? | |
| cinnamoyl-CoA + tyramine | - |
Papaver somniferum | CoA + cinnamoyltyramine | - |
? | |
| feruloyl-CoA + dopamine | - |
Papaver somniferum | CoA + feruloyldopamine | - |
? | |
| feruloyl-CoA + tyramine | - |
Papaver somniferum | CoA + feruloyltyramine | - |
? | |
| sinapoyl-CoA + tyramine | - |
Papaver somniferum | CoA + sinapoyltyramine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 25000, SDS-PAGE | Papaver somniferum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Papaver somniferum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | half-maximal activity at pH 6.5 and pH 9.5 | Papaver somniferum |
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