BRENDA - Enzyme Database show
show all sequences of 2.3.1.110

Purification, characterization, and immunolocalization of hydroxycinnamoyl-CoA: tyramine N-(hydroxycinnamoyl)transferase from opium poppy

Yu, M.; Facchini, P.J.; Planta 209, 33-44 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
almost complete inactivation at 1 mM
Papaver somniferum
FeSO4
almost complete inactivation at 1 mM
Papaver somniferum
ZnCl2
almost complete inactivation at 1 mM
Papaver somniferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
cinnamoyl-CoA
-
Papaver somniferum
0.017
-
4-Coumaroyl-CoA
-
Papaver somniferum
0.021
-
sinapoyl-CoA
-
Papaver somniferum
0.062
-
feruloyl-CoA
-
Papaver somniferum
0.076
-
tyramine
-
Papaver somniferum
0.078
-
dopamine
-
Papaver somniferum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 25000, SDS-PAGE
Papaver somniferum
50000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
feruloyl-CoA + tyramine
Papaver somniferum
-
CoA + feruloyltyramine
-
Papaver somniferum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
opium poppy
-
Purification (Commentary)
Commentary
Organism
988fold
Papaver somniferum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
anther
-
Papaver somniferum
-
root
xylem
Papaver somniferum
-
seed
-
Papaver somniferum
-
xylem
-
Papaver somniferum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.76
-
-
Papaver somniferum
Storage Stability
Storage Stability
Organism
-20C, no loss of activity for 2 months in the presence of 2-mercaptoethanol
Papaver somniferum
-80C, no loss of activity for 4 months in the presence of 2-mercaptoethanol
Papaver somniferum
4C, no loss of activity for 14 days in the presence of 2-mercaptoethanol
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + cinnamoyltyramine
-
-
-
?
feruloyl-CoA + dopamine
-
486134
Papaver somniferum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + feruloyltyramine
-
486134
Papaver somniferum
?
sinapoyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + sinapoyltyramine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 25000, SDS-PAGE
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Papaver somniferum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
half-maximal activity at pH 6.5 and pH 9.5
Papaver somniferum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
almost complete inactivation at 1 mM
Papaver somniferum
FeSO4
almost complete inactivation at 1 mM
Papaver somniferum
ZnCl2
almost complete inactivation at 1 mM
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
cinnamoyl-CoA
-
Papaver somniferum
0.017
-
4-Coumaroyl-CoA
-
Papaver somniferum
0.021
-
sinapoyl-CoA
-
Papaver somniferum
0.062
-
feruloyl-CoA
-
Papaver somniferum
0.076
-
tyramine
-
Papaver somniferum
0.078
-
dopamine
-
Papaver somniferum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 25000, SDS-PAGE
Papaver somniferum
50000
-
gel filtration
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
feruloyl-CoA + tyramine
Papaver somniferum
-
CoA + feruloyltyramine
-
Papaver somniferum
?
Purification (Commentary) (protein specific)
Commentary
Organism
988fold
Papaver somniferum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
anther
-
Papaver somniferum
-
root
xylem
Papaver somniferum
-
seed
-
Papaver somniferum
-
xylem
-
Papaver somniferum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.76
-
-
Papaver somniferum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, no loss of activity for 2 months in the presence of 2-mercaptoethanol
Papaver somniferum
-80C, no loss of activity for 4 months in the presence of 2-mercaptoethanol
Papaver somniferum
4C, no loss of activity for 14 days in the presence of 2-mercaptoethanol
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + cinnamoyltyramine
-
-
-
?
feruloyl-CoA + dopamine
-
486134
Papaver somniferum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + feruloyltyramine
-
486134
Papaver somniferum
?
sinapoyl-CoA + tyramine
-
486134
Papaver somniferum
CoA + sinapoyltyramine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 25000, SDS-PAGE
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Papaver somniferum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
half-maximal activity at pH 6.5 and pH 9.5
Papaver somniferum
Other publictions for EC 2.3.1.110
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736840
Campos
Transgenic tomato plants overe ...
Solanum lycopersicum
Mol. Plant Microbe Interact.
27
1159-1169
2014
-
-
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5
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1
1
-
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-
720789
Landmann
Substrate promiscuity of a ros ...
Lavandula angustifolia
Planta
234
305-320
2011
-
-
1
-
-
-
-
11
1
-
1
1
-
1
-
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1
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1
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1
8
1
1
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1
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1
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11
1
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1
8
1
1
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1
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702823
Kang
Production of plant-specific t ...
Capsicum annuum
Biotechnol. Lett.
31
1469-1475
2009
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1
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-
-
702831
Park
Endosperm-specific expression ...
Capsicum annuum
Biotechnol. Lett.
31
911-915
2009
-
1
1
-
1
-
-
-
-
-
-
-
-
4
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1
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1
1
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1
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-
673815
Niehl
-
Systemic Potato virus X infect ...
Solanum tuberosum
Funct. Plant Biol.
33
593-604
2006
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-
676598
Kang
Functional analysis of the ami ...
Capsicum annuum
Plant Physiol.
140
704-715
2006
-
-
1
-
3
-
-
8
-
-
1
-
-
1
-
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1
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-
6
1
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1
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-
3
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8
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1
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1
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6
1
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-
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-
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-
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-
660324
Hagel
Elevated tyrosine decarboxylas ...
Nicotiana tabacum
Planta
221
904-914
2005
-
-
-
-
-
-
-
-
-
-
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1
-
4
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1
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1
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1
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1
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-
660244
Jang
Production of coumaroylseroton ...
Capsicum annuum
Plant Physiol.
135
346-356
2004
-
-
-
-
-
-
1
9
-
-
-
-
-
3
-
-
1
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1
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-
7
-
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-
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1
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9
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1
-
1
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-
7
-
-
-
-
-
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-
-
-
-
-
486128
Back
Cloning and characterization o ...
Capsicum annuum
Plant Cell Physiol.
42
475-481
2001
-
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1
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-
1
1
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5
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1
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5
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6
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1
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1
1
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1
-
5
-
-
6
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
486131
Negrel
L-Tyrosine beta-naphthylamide ...
Nicotiana tabacum, Solanum tuberosum
Phytochemistry
56
523-527
2001
-
-
-
-
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8
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4
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2
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2
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8
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8
8
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2
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2
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-
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-
-
486130
Ishihara
Induction of N-hydroxycinnamoy ...
Zea mays
Biosci. Biotechnol. Biochem.
64
1025-1031
2000
-
-
-
-
-
-
2
8
-
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1
1
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2
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1
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1
1
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7
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2
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8
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1
1
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1
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1
1
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7
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-
-
-
-
-
-
-
-
-
-
486129
Farmer
Identification and characteriz ...
Nicotiana tabacum
Eur. J. Biochem.
263
686-694
1999
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1
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3
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2
1
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1
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1
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2
1
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1
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1
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2
1
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486133
Schmidt
Cloning and expression of a po ...
Solanum tuberosum
J. Biol. Chem.
274
4273-4280
1999
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1
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2
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2
1
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4
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9
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1
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1
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1
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9
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486134
Yu
Purification, characterization ...
Papaver somniferum
Planta
209
33-44
1999
-
-
-
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3
6
-
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2
1
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1
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1
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4
1
3
5
1
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1
1
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3
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6
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2
1
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1
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4
1
3
5
1
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1
1
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486132
Negrel
Purification, characterization ...
Nicotiana tabacum
Eur. J. Biochem.
247
1127-1135
1997
1
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11
12
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2
1
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2
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1
1
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1
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13
1
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9
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1
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11
9
12
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2
1
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1
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1
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13
1
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486127
Fleurence
-
Partial purification of tyrami ...
Nicotiana tabacum
Phytochemistry
28
733-736
1989
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2
2
4
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1
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1
1
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1
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1
1
2
6
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1
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1
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2
-
2
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4
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1
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1
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1
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1
1
2
6
-
1
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1
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-
-
486126
Negrel
-
The biosynthesis of feruloylty ...
Nicotiana tabacum
Phytochemistry
23
2797-2801
1984
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1
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6
1
1
1
1
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1
1
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1
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1
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1
14
-
1
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4
1
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-
1
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6
1
1
1
1
-
1
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1
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1
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1
14
-
1
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4
1
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