BRENDA - Enzyme Database show
show all sequences of 2.3.1.110

Purification, characterization and partial amino acid sequencing of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase from tobacco cell-suspension cultures

Negrel, J.; Javelle, F.; Eur. J. Biochem. 247, 1127-1135 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
addition of 14% leads to 2fold increase of enzyme activity
Nicotiana tabacum
Inhibitors
Inhibitors
Commentary
Organism
Structure
ammonium sulfate
20% activity at 0.5 M
Nicotiana tabacum
D-tyrosine methyl ester
-
Nicotiana tabacum
DL-tyrosine methyl ester
-
Nicotiana tabacum
L-tyrosine benzyl ester
irreversible binding to tyramine binding site
Nicotiana tabacum
L-tyrosine ethyl ester
-
Nicotiana tabacum
L-tyrosine methyl ester
-
Nicotiana tabacum
L-tyrosine-tert-butyl ester
-
Nicotiana tabacum
L-tyrosineamide
-
Nicotiana tabacum
L-tyrosinol
-
Nicotiana tabacum
N-Methyltyramine
-
Nicotiana tabacum
NaCl
20% activity at 0.5 M
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
feruloyl-CoA
-
Nicotiana tabacum
0.001
-
sinapoyl-CoA
-
Nicotiana tabacum
0.002
-
4-Coumaroyl-CoA
-
Nicotiana tabacum
0.002
-
isoferuloyl-CoA
-
Nicotiana tabacum
0.0043
-
tyramine
-
Nicotiana tabacum
0.0047
-
cinnamoyl-CoA
-
Nicotiana tabacum
0.0113
-
octopamine
-
Nicotiana tabacum
0.17
-
4-hydroxyphenylpropylamine
-
Nicotiana tabacum
0.202
-
dopamine
-
Nicotiana tabacum
1.57
-
noradrenaline
-
Nicotiana tabacum
1.585
-
3-methoxytyramine
-
Nicotiana tabacum
4.276
-
beta-phenylethylamine
-
Nicotiana tabacum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
SDS-PAGE
Nicotiana tabacum
48000
-
gel filtration
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
feruloyl-CoA + tyramine
Nicotiana tabacum
-
CoA + feruloyltyramine
-
Nicotiana tabacum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
-
cv. Xanthi
-
Purification (Commentary)
Commentary
Organism
homogeneity
Nicotiana tabacum
Reaction
Reaction
Commentary
Organism
feruloyl-CoA + tyramine = CoA + N-feruloyltyramine
mechanism
Nicotiana tabacum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2
-
-
Nicotiana tabacum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + N-caffeoyltyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + cinnamoyltyramine
-
-
-
?
feruloyl-CoA + (4-hydroxyphenyl)propylamine
-
486132
Nicotiana tabacum
CoA + feruloyl-(4-hydroxyphenyl)propylamine
-
-
-
?
feruloyl-CoA + 3-methoxytyramine
-
486132
Nicotiana tabacum
CoA + feruloyl-3-methoxytyramine
-
-
-
?
feruloyl-CoA + beta-phenylethylamine
-
486132
Nicotiana tabacum
CoA + feruloyl-beta-phenylethylamine
-
-
-
?
feruloyl-CoA + dopamine
-
486132
Nicotiana tabacum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + noradrenaline
-
486132
Nicotiana tabacum
CoA + feruloylnoradrenaline
-
-
-
?
feruloyl-CoA + octopamine
-
486132
Nicotiana tabacum
CoA + feruloyloctopamine
-
-
-
?
feruloyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + feruloyltyramine
-
486132
Nicotiana tabacum
?
feruloyl-CoA + tyramine
low specificity for cinnamoyl-CoA derivates and hydroxyphenethylamines
486132
Nicotiana tabacum
CoA + feruloyltyramine
-
486132
Nicotiana tabacum
?
isoferuloyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + isoferuloyltyramine
-
-
-
?
sinapoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + sinapoyltyramine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 24000, SDS-PAGE
Nicotiana tabacum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.003
-
L-tyrosine benzyl ester
-
Nicotiana tabacum
0.018
-
L-tyrosine-tert-butyl ester
-
Nicotiana tabacum
0.02
-
L-tyrosine ethyl ester
-
Nicotiana tabacum
0.026
-
L-tyrosine methyl ester
-
Nicotiana tabacum
0.046
-
L-tyrosinol
-
Nicotiana tabacum
0.055
-
DL-tyrosine methyl ester
-
Nicotiana tabacum
0.58
-
D-tyrosine methyl ester
-
Nicotiana tabacum
2
-
L-tyrosineamide
-
Nicotiana tabacum
2.2
-
N-Methyltyramine
-
Nicotiana tabacum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
addition of 14% leads to 2fold increase of enzyme activity
Nicotiana tabacum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ammonium sulfate
20% activity at 0.5 M
Nicotiana tabacum
D-tyrosine methyl ester
-
Nicotiana tabacum
DL-tyrosine methyl ester
-
Nicotiana tabacum
L-tyrosine benzyl ester
irreversible binding to tyramine binding site
Nicotiana tabacum
L-tyrosine ethyl ester
-
Nicotiana tabacum
L-tyrosine methyl ester
-
Nicotiana tabacum
L-tyrosine-tert-butyl ester
-
Nicotiana tabacum
L-tyrosineamide
-
Nicotiana tabacum
L-tyrosinol
-
Nicotiana tabacum
N-Methyltyramine
-
Nicotiana tabacum
NaCl
20% activity at 0.5 M
Nicotiana tabacum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.003
-
L-tyrosine benzyl ester
-
Nicotiana tabacum
0.018
-
L-tyrosine-tert-butyl ester
-
Nicotiana tabacum
0.02
-
L-tyrosine ethyl ester
-
Nicotiana tabacum
0.026
-
L-tyrosine methyl ester
-
Nicotiana tabacum
0.046
-
L-tyrosinol
-
Nicotiana tabacum
0.055
-
DL-tyrosine methyl ester
-
Nicotiana tabacum
0.58
-
D-tyrosine methyl ester
-
Nicotiana tabacum
2
-
L-tyrosineamide
-
Nicotiana tabacum
2.2
-
N-Methyltyramine
-
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
feruloyl-CoA
-
Nicotiana tabacum
0.001
-
sinapoyl-CoA
-
Nicotiana tabacum
0.002
-
4-Coumaroyl-CoA
-
Nicotiana tabacum
0.002
-
isoferuloyl-CoA
-
Nicotiana tabacum
0.0043
-
tyramine
-
Nicotiana tabacum
0.0047
-
cinnamoyl-CoA
-
Nicotiana tabacum
0.0113
-
octopamine
-
Nicotiana tabacum
0.17
-
4-hydroxyphenylpropylamine
-
Nicotiana tabacum
0.202
-
dopamine
-
Nicotiana tabacum
1.57
-
noradrenaline
-
Nicotiana tabacum
1.585
-
3-methoxytyramine
-
Nicotiana tabacum
4.276
-
beta-phenylethylamine
-
Nicotiana tabacum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
SDS-PAGE
Nicotiana tabacum
48000
-
gel filtration
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
feruloyl-CoA + tyramine
Nicotiana tabacum
-
CoA + feruloyltyramine
-
Nicotiana tabacum
?
Purification (Commentary) (protein specific)
Commentary
Organism
homogeneity
Nicotiana tabacum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2
-
-
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + N-caffeoyltyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + cinnamoyltyramine
-
-
-
?
feruloyl-CoA + (4-hydroxyphenyl)propylamine
-
486132
Nicotiana tabacum
CoA + feruloyl-(4-hydroxyphenyl)propylamine
-
-
-
?
feruloyl-CoA + 3-methoxytyramine
-
486132
Nicotiana tabacum
CoA + feruloyl-3-methoxytyramine
-
-
-
?
feruloyl-CoA + beta-phenylethylamine
-
486132
Nicotiana tabacum
CoA + feruloyl-beta-phenylethylamine
-
-
-
?
feruloyl-CoA + dopamine
-
486132
Nicotiana tabacum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + noradrenaline
-
486132
Nicotiana tabacum
CoA + feruloylnoradrenaline
-
-
-
?
feruloyl-CoA + octopamine
-
486132
Nicotiana tabacum
CoA + feruloyloctopamine
-
-
-
?
feruloyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + feruloyltyramine
-
486132
Nicotiana tabacum
?
feruloyl-CoA + tyramine
low specificity for cinnamoyl-CoA derivates and hydroxyphenethylamines
486132
Nicotiana tabacum
CoA + feruloyltyramine
-
486132
Nicotiana tabacum
?
isoferuloyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + isoferuloyltyramine
-
-
-
?
sinapoyl-CoA + tyramine
-
486132
Nicotiana tabacum
CoA + sinapoyltyramine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 24000, SDS-PAGE
Nicotiana tabacum
Other publictions for EC 2.3.1.110
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736840
Campos
Transgenic tomato plants overe ...
Solanum lycopersicum
Mol. Plant Microbe Interact.
27
1159-1169
2014
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5
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1
1
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720789
Landmann
Substrate promiscuity of a ros ...
Lavandula angustifolia
Planta
234
305-320
2011
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1
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11
1
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1
1
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1
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1
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8
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1
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702823
Kang
Production of plant-specific t ...
Capsicum annuum
Biotechnol. Lett.
31
1469-1475
2009
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702831
Park
Endosperm-specific expression ...
Capsicum annuum
Biotechnol. Lett.
31
911-915
2009
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1
1
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1
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673815
Niehl
-
Systemic Potato virus X infect ...
Solanum tuberosum
Funct. Plant Biol.
33
593-604
2006
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676598
Kang
Functional analysis of the ami ...
Capsicum annuum
Plant Physiol.
140
704-715
2006
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1
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3
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8
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1
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1
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1
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6
1
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1
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3
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1
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1
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6
1
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660324
Hagel
Elevated tyrosine decarboxylas ...
Nicotiana tabacum
Planta
221
904-914
2005
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4
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660244
Jang
Production of coumaroylseroton ...
Capsicum annuum
Plant Physiol.
135
346-356
2004
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1
9
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3
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1
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1
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7
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1
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9
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7
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486128
Back
Cloning and characterization o ...
Capsicum annuum
Plant Cell Physiol.
42
475-481
2001
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1
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1
1
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5
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1
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5
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6
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1
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1
1
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1
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5
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6
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-
-
-
-
-
-
-
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486131
Negrel
L-Tyrosine beta-naphthylamide ...
Nicotiana tabacum, Solanum tuberosum
Phytochemistry
56
523-527
2001
-
-
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8
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4
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2
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2
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8
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8
8
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2
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2
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-
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486130
Ishihara
Induction of N-hydroxycinnamoy ...
Zea mays
Biosci. Biotechnol. Biochem.
64
1025-1031
2000
-
-
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2
8
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1
1
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2
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1
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1
1
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7
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2
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8
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1
1
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1
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1
1
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7
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486129
Farmer
Identification and characteriz ...
Nicotiana tabacum
Eur. J. Biochem.
263
686-694
1999
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1
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3
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2
1
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1
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1
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2
1
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1
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3
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2
1
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1
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2
1
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486133
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486134
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Purification, characterization ...
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486132
Negrel
Purification, characterization ...
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1997
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486127
Fleurence
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Partial purification of tyrami ...
Nicotiana tabacum
Phytochemistry
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733-736
1989
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486126
Negrel
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The biosynthesis of feruloylty ...
Nicotiana tabacum
Phytochemistry
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2797-2801
1984
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