BRENDA - Enzyme Database
show all sequences of 2.3.1.108

Tubulin acetyltransferase discovered: ciliary role in the ancestral eukaryote expanded to neurons in metazoans

Leroux, M.; Proc. Natl. Acad. Sci. USA 107, 21238-21239 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
genes alphaTAT/MEC-17, phylogenetic analysis
Caenorhabditis elegans
genes alphaTAT/MEC-17, phylogenetic analysis
Homo sapiens
genes alphaTAT/MEC-17, phylogenetic analysis
Tetrahymena sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
Homo sapiens
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Caenorhabditis elegans
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Tetrahymena sp.
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
additional information
Homo sapiens
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
additional information
Caenorhabditis elegans
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
additional information
Tetrahymena sp.
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caenorhabditis elegans
-
-
-
Homo sapiens
-
-
-
Tetrahymena sp.
-
gene MEC17
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
most Caenorhabditis elegans-ciliated sensory neurons lack detectable acetylated tubulin
Caenorhabditis elegans
-
neuron
MEC-12 is present in touch receptor neurons and other (ciliated) neurons. mec-17 expression is limited to touch receptor neurons, its paralogue atat-2 is also expressed in some ciliated neurons
Caenorhabditis elegans
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Caenorhabditis elegans
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Tetrahymena sp.
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Homo sapiens
?
-
-
-
-
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Caenorhabditis elegans
?
-
-
-
-
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Tetrahymena sp.
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Homo sapiens
acetyl-CoA
-
Tetrahymena sp.
acetyl-CoA
-
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
Commentary
Organism
genes alphaTAT/MEC-17, phylogenetic analysis
Caenorhabditis elegans
genes alphaTAT/MEC-17, phylogenetic analysis
Homo sapiens
genes alphaTAT/MEC-17, phylogenetic analysis
Tetrahymena sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Homo sapiens
acetyl-CoA
-
Tetrahymena sp.
acetyl-CoA
-
Caenorhabditis elegans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
Homo sapiens
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Caenorhabditis elegans
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Tetrahymena sp.
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
additional information
Homo sapiens
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
additional information
Caenorhabditis elegans
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
additional information
Tetrahymena sp.
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
most Caenorhabditis elegans-ciliated sensory neurons lack detectable acetylated tubulin
Caenorhabditis elegans
-
neuron
MEC-12 is present in touch receptor neurons and other (ciliated) neurons. mec-17 expression is limited to touch receptor neurons, its paralogue atat-2 is also expressed in some ciliated neurons
Caenorhabditis elegans
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Caenorhabditis elegans
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
720906
Tetrahymena sp.
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Homo sapiens
?
-
-
-
-
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Caenorhabditis elegans
?
-
-
-
-
additional information
alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
720906
Tetrahymena sp.
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Caenorhabditis elegans
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Homo sapiens
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Tetrahymena sp.
malfunction
loss of Caenorhabditis elegans alphaTAT activity in the mec-17, atat-2 double mutant affects touch receptor neuron mechanosensation to a greater extent than having a nonacetylatable MEC-12 (K40R) form of tubulin in the organism
Caenorhabditis elegans
malfunction
a delay in cilium formation for cultured human cells depleted of alphaTAT/MEC-17
Homo sapiens
malfunction
disrupting MEC17 in Tetrahymena abolishes tubulin acetylation, but no overt defect in cilium formation or motility occurs
Tetrahymena sp.
physiological function
MEC-17 and ATAT-2 are required for body touch sensation, which depends on the nonciliated, acetylated tubulin-containing touch receptor neurons. ATAT-2 is needed for acetylating tubulin in dendritic processes and cilia in those neurons. Additional role(s) for the acetyltransferase independent of tubulin acetylation
Caenorhabditis elegans
General Information (protein specific)
General Information
Commentary
Organism
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Caenorhabditis elegans
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Homo sapiens
evolution
distribution of alphaTAT/MEC-17 across all eukaryotic clades reveals that it was present in the last eukaryotic common ancestor, which was ciliated
Tetrahymena sp.
malfunction
loss of Caenorhabditis elegans alphaTAT activity in the mec-17, atat-2 double mutant affects touch receptor neuron mechanosensation to a greater extent than having a nonacetylatable MEC-12 (K40R) form of tubulin in the organism
Caenorhabditis elegans
malfunction
a delay in cilium formation for cultured human cells depleted of alphaTAT/MEC-17
Homo sapiens
malfunction
disrupting MEC17 in Tetrahymena abolishes tubulin acetylation, but no overt defect in cilium formation or motility occurs
Tetrahymena sp.
physiological function
MEC-17 and ATAT-2 are required for body touch sensation, which depends on the nonciliated, acetylated tubulin-containing touch receptor neurons. ATAT-2 is needed for acetylating tubulin in dendritic processes and cilia in those neurons. Additional role(s) for the acetyltransferase independent of tubulin acetylation
Caenorhabditis elegans
Other publictions for EC 2.3.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736728
Nakakura
Intracellular localization of ...
Rattus norvegicus
Med. Mol. Morphol.
49
133-143
2016
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735638
Yuzawa
Structural basis of cofactor-m ...
Homo sapiens
Biochem. J.
467
103-113
2015
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2
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735908
Neumann
Loss of MEC-17 leads to microt ...
Caenorhabditis elegans
Cell Rep.
6
93-103
2014
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1
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735916
Szyk
Molecular basis for age-depend ...
Homo sapiens
Cell
157
1405-1415
2014
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1
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736637
Davenport
Structural and functional char ...
Homo sapiens
J. Mol. Biol.
426
2605-2616
2014
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1
33
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20
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3
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18
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33
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18
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19
19
736778
Howes
Effects of tubulin acetylation ...
Homo sapiens
Mol. Biol. Cell
25
257-266
2014
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736872
Wang
Microtubule acetylation amplif ...
Mus musculus
Nat. Commun.
5
3479
2014
1
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735631
Yang
Regulation of adipogenesis by ...
Mus musculus
Biochem. J.
449
605-612
2013
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1
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736795
Kalebic
Tubulin acetyltransferase alph ...
Mus musculus
Mol. Cell. Biol.
33
1114-1123
2013
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736870
Kalebic
alphaTAT1 is the major alpha-t ...
Mus musculus
Nat. Commun.
4
1962
2013
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736879
Montagnac
alphaTAT1 catalyses microtubul ...
Homo sapiens
Nature
502
567-570
2013
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719320
Topalidou
Genetically separable function ...
Caenorhabditis elegans
Curr. Biol.
22
1057-1065
2012
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719403
Castro-Castro
ATAT1/MEC-17 acetyltransferase ...
Homo sapiens
Eur. J. Cell Biol.
91
950-960
2012
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706413
Solinger
The Caenorhabditis elegans elo ...
Caenorhabditis elegans
PLoS Genet.
6
e1000820
2010
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720540
Akella
MEC-17 is an alpha-tubulin ace ...
Caenorhabditis elegans, Chlamydomonas reinhardtii, Danio rerio, Mus musculus, Tetrahymena thermophila
Nature
467
218-222
2010
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720906
Leroux
Tubulin acetyltransferase disc ...
Caenorhabditis elegans, Homo sapiens, Tetrahymena sp.
Proc. Natl. Acad. Sci. USA
107
21238-21239
2010
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720907
Shida
The major alpha-tubulin K40 ac ...
Caenorhabditis elegans, no activity in Potorous tridactylis
Proc. Natl. Acad. Sci. USA
107
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