BRENDA - Enzyme Database
show all sequences of 2.3.1.108

ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion

Castro-Castro, A.; Janke, C.; Montagnac, G.; Paul-Gilloteaux, P.; Chavrier, P.; Eur. J. Cell Biol. 91, 950-960 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
ATAT1, DNA and amino acid sequence determination and analysis, stable functional expression of EGFP-ATAT1 and mutant ATAT1D157N in MDA-MB-231 cells and in HeLa cells
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
D157N
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
additional information
overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + cortactin
Homo sapiens
ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
CoA + N-acetyl-cortactin
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Homo sapiens
acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
MDA-MB-231 cell
invasive breast cancer cells
Homo sapiens
-
additional information
ATAT1 colocalizes with cortactin at the adherent surface of the cells, association of ATAT1 and cortactin at invadopodia, subcellular localization study, overview
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + cortactin
-
719403
Homo sapiens
CoA + N-acetyl-cortactin
-
-
-
?
acetyl-CoA + cortactin
ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
719403
Homo sapiens
CoA + N-acetyl-cortactin
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
719403
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
719403
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
ATAT1, DNA and amino acid sequence determination and analysis, stable functional expression of EGFP-ATAT1 and mutant ATAT1D157N in MDA-MB-231 cells and in HeLa cells
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D157N
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
additional information
overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + cortactin
Homo sapiens
ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
CoA + N-acetyl-cortactin
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
Homo sapiens
acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
MDA-MB-231 cell
invasive breast cancer cells
Homo sapiens
-
additional information
ATAT1 colocalizes with cortactin at the adherent surface of the cells, association of ATAT1 and cortactin at invadopodia, subcellular localization study, overview
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + cortactin
-
719403
Homo sapiens
CoA + N-acetyl-cortactin
-
-
-
?
acetyl-CoA + cortactin
ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
719403
Homo sapiens
CoA + N-acetyl-cortactin
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
719403
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
719403
Homo sapiens
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin. Random invasive migration of cells in 3D collagen I is inhibited by 40-50% upon MT1-MMP or ATAT1 knockdown
Homo sapiens
metabolism
a balance of acetylation and deaceylation by ATAT1/HDAC6, histone deacetylase 6, enzymes with opposite activities regulates the migratory and invasive capacities of breast tumor cells
Homo sapiens
physiological function
dynamics and distribution of MT1-MMP-positive endosomes require regulation of acetylation levels, ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. Acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen. ATAT1 colocalizes with cortactin at the adherent surface of the cells and it is required for 2D migration and invasive migration of MDA-MB-231 cells in collagen matrix
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin. Random invasive migration of cells in 3D collagen I is inhibited by 40-50% upon MT1-MMP or ATAT1 knockdown
Homo sapiens
metabolism
a balance of acetylation and deaceylation by ATAT1/HDAC6, histone deacetylase 6, enzymes with opposite activities regulates the migratory and invasive capacities of breast tumor cells
Homo sapiens
physiological function
dynamics and distribution of MT1-MMP-positive endosomes require regulation of acetylation levels, ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. Acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen. ATAT1 colocalizes with cortactin at the adherent surface of the cells and it is required for 2D migration and invasive migration of MDA-MB-231 cells in collagen matrix
Homo sapiens
Other publictions for EC 2.3.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736728
Nakakura
Intracellular localization of ...
Rattus norvegicus
Med. Mol. Morphol.
49
133-143
2016
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735638
Yuzawa
Structural basis of cofactor-m ...
Homo sapiens
Biochem. J.
467
103-113
2015
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1
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735908
Neumann
Loss of MEC-17 leads to microt ...
Caenorhabditis elegans
Cell Rep.
6
93-103
2014
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735916
Szyk
Molecular basis for age-depend ...
Homo sapiens
Cell
157
1405-1415
2014
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1
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1
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736637
Davenport
Structural and functional char ...
Homo sapiens
J. Mol. Biol.
426
2605-2616
2014
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1
33
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20
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3
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1
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18
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1
33
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20
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18
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19
19
736778
Howes
Effects of tubulin acetylation ...
Homo sapiens
Mol. Biol. Cell
25
257-266
2014
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736872
Wang
Microtubule acetylation amplif ...
Mus musculus
Nat. Commun.
5
3479
2014
1
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735631
Yang
Regulation of adipogenesis by ...
Mus musculus
Biochem. J.
449
605-612
2013
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1
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736795
Kalebic
Tubulin acetyltransferase alph ...
Mus musculus
Mol. Cell. Biol.
33
1114-1123
2013
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1
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1
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5
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1
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736870
Kalebic
alphaTAT1 is the major alpha-t ...
Mus musculus
Nat. Commun.
4
1962
2013
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5
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1
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736879
Montagnac
alphaTAT1 catalyses microtubul ...
Homo sapiens
Nature
502
567-570
2013
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719320
Topalidou
Genetically separable function ...
Caenorhabditis elegans
Curr. Biol.
22
1057-1065
2012
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2
2
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719403
Castro-Castro
ATAT1/MEC-17 acetyltransferase ...
Homo sapiens
Eur. J. Cell Biol.
91
950-960
2012
-
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1
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2
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3
3
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706413
Solinger
The Caenorhabditis elegans elo ...
Caenorhabditis elegans
PLoS Genet.
6
e1000820
2010
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720540
Akella
MEC-17 is an alpha-tubulin ace ...
Caenorhabditis elegans, Chlamydomonas reinhardtii, Danio rerio, Mus musculus, Tetrahymena thermophila
Nature
467
218-222
2010
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13
13
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720906
Leroux
Tubulin acetyltransferase disc ...
Caenorhabditis elegans, Homo sapiens, Tetrahymena sp.
Proc. Natl. Acad. Sci. USA
107
21238-21239
2010
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3
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3
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7
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720907
Shida
The major alpha-tubulin K40 ac ...
Caenorhabditis elegans, no activity in Potorous tridactylis
Proc. Natl. Acad. Sci. USA
107
21517-21522
2010
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1
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6
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3
3
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486115
MacRae
Tubulin post-translational mod ...
Bos taurus, Chlamydomonas reinhardtii
Eur. J. Biochem.
244
265-278
1997
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1
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2
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2
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1
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1
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486116
Lloyd
Assay of tubulin acetyltransfe ...
Bos taurus
Anal. Biochem.
216
42-46
1994
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486117
Maruta
The acetylation of alpha-tubul ...
Chlamydomonas reinhardtii
J. Cell Biol.
103
571-579
1986
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486118
Greer
Alpha-tubulin acetylase activi ...
Chlamydomonas reinhardtii 21 gr, Chlamydomonas reinhardtii
J. Cell Biol.
101
2081-2084
1985
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