BRENDA - Enzyme Database
show all sequences of 2.3.1.108

Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization

Topalidou, I.; Keller, C.; Kalebic, N.; Nguyen, K.C.; Somhegyi, H.; Politi, K.A.; Heppenstall, P.; Hall, D.H.; Chalfie, M.; Curr. Biol. 22, 1057-1065 (2012)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
Caenorhabditis elegans
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caenorhabditis elegans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
-
719320
Caenorhabditis elegans
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Caenorhabditis elegans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
Caenorhabditis elegans
-
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [alpha-tubulin]-L-lysine
-
719320
Caenorhabditis elegans
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
touch receptor neurons in mec-17 but not atat-2 mutants exhibit morphological defects, phenotype, overview. Both mec-17(ok2109) and mec-17(u265) produced an increase in the length of the touch receptor neuron processes, effects on touch receptor neuron microtubule organization and structure, overview. Enzymatically inactive MEC-17 mutants rescue some of the mec-17 phenotypes, the function of mec-17 is protein-dependent
Caenorhabditis elegans
physiological function
the enzymatic activity of the alpha-tubulin acetyltransferase MEC-17 allows the production of 15-p microtubules in the touch receptor neurons microtubules, specific role for alpha-TAT in the formation of microtubules and in the production of higher order microtubules arrays. The alpha-TAT protein has functions that require acetyltransferase activity, such as the determination of protofilament number, and others that do not, e.g. presence of internal microtubule structures. Mec-17 and atat-2 are needed for touch sensitivity and MEC-12 acetylation
Caenorhabditis elegans
General Information (protein specific)
General Information
Commentary
Organism
malfunction
touch receptor neurons in mec-17 but not atat-2 mutants exhibit morphological defects, phenotype, overview. Both mec-17(ok2109) and mec-17(u265) produced an increase in the length of the touch receptor neuron processes, effects on touch receptor neuron microtubule organization and structure, overview. Enzymatically inactive MEC-17 mutants rescue some of the mec-17 phenotypes, the function of mec-17 is protein-dependent
Caenorhabditis elegans
physiological function
the enzymatic activity of the alpha-tubulin acetyltransferase MEC-17 allows the production of 15-p microtubules in the touch receptor neurons microtubules, specific role for alpha-TAT in the formation of microtubules and in the production of higher order microtubules arrays. The alpha-TAT protein has functions that require acetyltransferase activity, such as the determination of protofilament number, and others that do not, e.g. presence of internal microtubule structures. Mec-17 and atat-2 are needed for touch sensitivity and MEC-12 acetylation
Caenorhabditis elegans
Other publictions for EC 2.3.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736728
Nakakura
Intracellular localization of ...
Rattus norvegicus
Med. Mol. Morphol.
49
133-143
2016
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Yuzawa
Structural basis of cofactor-m ...
Homo sapiens
Biochem. J.
467
103-113
2015
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735908
Neumann
Loss of MEC-17 leads to microt ...
Caenorhabditis elegans
Cell Rep.
6
93-103
2014
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735916
Szyk
Molecular basis for age-depend ...
Homo sapiens
Cell
157
1405-1415
2014
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736637
Davenport
Structural and functional char ...
Homo sapiens
J. Mol. Biol.
426
2605-2616
2014
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33
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736778
Howes
Effects of tubulin acetylation ...
Homo sapiens
Mol. Biol. Cell
25
257-266
2014
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736872
Wang
Microtubule acetylation amplif ...
Mus musculus
Nat. Commun.
5
3479
2014
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735631
Yang
Regulation of adipogenesis by ...
Mus musculus
Biochem. J.
449
605-612
2013
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1
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736795
Kalebic
Tubulin acetyltransferase alph ...
Mus musculus
Mol. Cell. Biol.
33
1114-1123
2013
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1
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1
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736870
Kalebic
alphaTAT1 is the major alpha-t ...
Mus musculus
Nat. Commun.
4
1962
2013
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1
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736879
Montagnac
alphaTAT1 catalyses microtubul ...
Homo sapiens
Nature
502
567-570
2013
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719320
Topalidou
Genetically separable function ...
Caenorhabditis elegans
Curr. Biol.
22
1057-1065
2012
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2
2
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719403
Castro-Castro
ATAT1/MEC-17 acetyltransferase ...
Homo sapiens
Eur. J. Cell Biol.
91
950-960
2012
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3
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706413
Solinger
The Caenorhabditis elegans elo ...
Caenorhabditis elegans
PLoS Genet.
6
e1000820
2010
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720540
Akella
MEC-17 is an alpha-tubulin ace ...
Caenorhabditis elegans, Chlamydomonas reinhardtii, Danio rerio, Mus musculus, Tetrahymena thermophila
Nature
467
218-222
2010
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720906
Leroux
Tubulin acetyltransferase disc ...
Caenorhabditis elegans, Homo sapiens, Tetrahymena sp.
Proc. Natl. Acad. Sci. USA
107
21238-21239
2010
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3
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7
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720907
Shida
The major alpha-tubulin K40 ac ...
Caenorhabditis elegans, no activity in Potorous tridactylis
Proc. Natl. Acad. Sci. USA
107
21517-21522
2010
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486115
MacRae
Tubulin post-translational mod ...
Bos taurus, Chlamydomonas reinhardtii
Eur. J. Biochem.
244
265-278
1997
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486116
Lloyd
Assay of tubulin acetyltransfe ...
Bos taurus
Anal. Biochem.
216
42-46
1994
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486117
Maruta
The acetylation of alpha-tubul ...
Chlamydomonas reinhardtii
J. Cell Biol.
103
571-579
1986
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486118
Greer
Alpha-tubulin acetylase activi ...
Chlamydomonas reinhardtii 21 gr, Chlamydomonas reinhardtii
J. Cell Biol.
101
2081-2084
1985
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