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Literature summary for 2.3.1.107 extracted from

  • Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.
    Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus (2010), J. Plant Physiol., 168, 549-557.
    View publication on PubMed
Search for more literature for 2.3.1.107

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker Catharanthus roseus

Protein Variants

Protein Variants Comment Organism
additional information analysis of interaction of vindoline biosynthetic enzymes including DAT using yeast two-hybrid assays, overview. DAT does not interact with the other enzymes Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Catharanthus roseus 5737
-
additional information GFP-tagged DAT is localized to the cytoplasm and the nucleus Catharanthus roseus
-
-
nucleus
-
 Catharanthus roseus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + deacetylvindoline Catharanthus roseus
-
 CoA + vindoline
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + deacetylvindoline
-
 Catharanthus roseus CoA + vindoline
-
 ?

Subunits

Subunits Comment Organism
More the enzyme is no hetero- or homodimer Catharanthus roseus

Synonyms

Synonyms Comment Organism
DAT
-
 Catharanthus roseus
deacetylvindoline-4-O-acetyltransferase
-
 Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Catharanthus roseus

General Information

General Information Comment Organism
metabolism vindoline constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial tabersonine-to-vindoline biosynthesis organization involving four enzymes for the six-step conversion, overview. Deacetylvindoline-4-O-acetyltransferase catalyzes teh last step of the pathway Catharanthus roseus