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Literature summary for 2.3.1.103 extracted from

  • Dahlbender, B.; Strack, D.
    Purification and properties of 1-(hydroxycinnamoyl)-glucose:1-(hydroxycinnamoyl)-glucose hydroxycinnamoyl-transferase from radish seedlings (1986), Phytochemistry, 25, 1043-1046.
No PubMed abstract available
Search for more literature for 2.3.1.103

Activating Compound

Activating Compound Comment Organism Structure
additional information no requirement for divalent cations or thiols Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, total inhibition Raphanus sativus
Ca2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 45% remaining activity Raphanus sativus
Co2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, total inhibition Raphanus sativus
dithiothreitol 1 mM, total inhibition Raphanus sativus
Mg2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 65% remaining activity Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 1-feruloyl-beta-D-glucose
-
 Raphanus sativus
0.42
-
 1-sinapoyl-beta-D-glucose
-
 Raphanus sativus
0.58
-
 1-(p-coumaroyl)-glucose
-
 Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration, SDS-PAGE Raphanus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose Raphanus sativus
-
 1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
-
 ir

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 L. var. sativus cv. Saxa
-

Purification (Commentary)

Purification (Comment) Organism
partial Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon dark grown seedlings show higher activity than light grown seedlings Raphanus sativus
-

Storage Stability

Storage Stability Organism
-20°C, presence of 3 mg/ml bovine serum albumin, at least 1 year Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(p-coumaroyl)-beta-D-glucose + 1-(p-coumaroyl)-beta-D-glucose
-
 Raphanus sativus 1,2-di-O-p-coumaroyl-beta-D-glucopyranose + D-glucose
-
 ir
1-feruloyl-beta-D-glucose + 1-feruloyl-beta-D-glucose
-
 Raphanus sativus 1,2-di-O-feruloyl-beta-glucopyranose + glucose
-
 ir
1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
-
 Raphanus sativus 1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
-
 ir
additional information strict specificity of acyl transfer to the C2 hydroxyl group of the acceptor glucose molecule Raphanus sativus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Raphanus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 HEPES-buffer Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 50% of maximal activity at pH 7.0 and pH 9.0 Raphanus sativus