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Literature summary for 2.3.1.101 extracted from
- Tetrahydromethanopterin-specific enzymes from Methanopyrus kandleri (2001), Methods Enzymol., 331, 317-353.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
formylmethanofuran | - |
Methanopyrus kandleri |  |
| 0.1 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| salts | stimulation of activity by salt 1000fold | Methanopyrus kandleri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000 | Methanopyrus kandleri |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | enzyme completely soluble in 80% ammonium sulfate | Methanopyrus kandleri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanopyrus kandleri | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| inactivated slowly under oxic conditions, purification in anaerobic chamber | Methanopyrus kandleri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| in a anaerobic chamber | Methanopyrus kandleri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 800 | - |
- |
Methanopyrus kandleri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000 | Methanopyrus kandleri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Methanopyrus kandleri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
stable up to, in presence of salts | Methanopyrus kandleri |
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Release 2023.1 (January 2023)
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