Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, slightly stimulates | Rattus norvegicus | |
Nonidet P-40 | stimulates at 0.05% | Rattus norvegicus | |
octyl glucoside | slight stimulation | Rattus norvegicus | |
sodium deoxycholate | stimulates at 0.5% | Rattus norvegicus | |
Triton X-100 | 0.1%, stimulates | Rattus norvegicus | |
Tween 20 | 0.05%, slight stimulation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium dodecyl sulfate | at concentration 0.5%: 92% loss of activity | Rattus norvegicus | |
Tween 20 | inhibits slightly at 0.5% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
palmitoyl-CoA | - |
Rattus norvegicus | |
0.046 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
0.05 | - |
stearoyl-CoA | - |
Rattus norvegicus | |
0.219 | - |
myristoyl-CoA | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 2 mM, stimulates | Rattus norvegicus | |
MnCl2 | stimulates | Rattus norvegicus | |
NaF | slight stimulation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | enzyme is probably involved in the acylation of endogenous myelin proteolipid protein | ? | - |
? | |
palmitoyl-CoA + myelin proteolipid protein | Rattus norvegicus | - |
CoA + myelin proteolipid O-palmitoylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain myelin | labeled product also found in vitro in brain tissue slices | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
freezing and thawing causes marked decrease in activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is probably involved in the acylation of endogenous myelin proteolipid protein | Rattus norvegicus | ? | - |
? | |
myristoyl-CoA + myelin proteolipid protein | low activity | Rattus norvegicus | CoA + myelin proteolipid O-myristoylprotein | - |
? | |
oleoyl-CoA + myelin proteolipid protein | - |
Rattus norvegicus | CoA + myelin proteolipid O-oleoylprotein | - |
? | |
palmitoyl-CoA + myelin proteolipid protein | - |
Rattus norvegicus | CoA + myelin proteolipid O-palmitoylprotein | - |
? | |
stearoyl-CoA + myelin proteolipid protein | - |
Rattus norvegicus | CoA + myelin proteolipid O-stearoylprotein | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 45% of maximal acticity at 10°C, 75% of maximal activity at 50°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
5 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 25% of maximal activity at pH 6, 90% of maximal activity at pH 8 | Rattus norvegicus |