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show all sequences of 2.3.1.100

Acylation of endogenous myelin proteolipid protein with different acyl-CoAs

Bizzozero, O.A.; McGarry, J.F.; Lees, M.B.; J. Biol. Chem. 262, 2138-2145 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
1 mM, slightly stimulates
Rattus norvegicus
Nonidet P-40
stimulates at 0.05%
Rattus norvegicus
octyl glucoside
slight stimulation
Rattus norvegicus
sodium deoxycholate
stimulates at 0.5%
Rattus norvegicus
Triton X-100
0.1%, stimulates
Rattus norvegicus
Tween 20
0.05%, slight stimulation
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Sodium dodecyl sulfate
at concentration 0.5%: 92% loss of activity
Rattus norvegicus
Tween 20
inhibits slightly at 0.5%
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
palmitoyl-CoA
-
Rattus norvegicus
0.046
-
oleoyl-CoA
-
Rattus norvegicus
0.05
-
stearoyl-CoA
-
Rattus norvegicus
0.219
-
myristoyl-CoA
-
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
MgCl2
2 mM, stimulates
Rattus norvegicus
MnCl2
stimulates
Rattus norvegicus
NaF
slight stimulation
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rattus norvegicus
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
?
-
-
-
palmitoyl-CoA + myelin proteolipid protein
Rattus norvegicus
-
CoA + myelin proteolipid O-palmitoylprotein
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain myelin
labeled product also found in vitro in brain tissue slices
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
freezing and thawing causes marked decrease in activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
486083
Rattus norvegicus
?
-
-
-
-
myristoyl-CoA + myelin proteolipid protein
low activity
486083
Rattus norvegicus
CoA + myelin proteolipid O-myristoylprotein
-
-
-
?
oleoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-oleoylprotein
-
-
-
?
palmitoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-palmitoylprotein
-
-
-
?
palmitoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-palmitoylprotein
-
486083
Rattus norvegicus
?
stearoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-stearoylprotein
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
50
45% of maximal acticity at 10C, 75% of maximal activity at 50C
Rattus norvegicus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
75
-
5 min, complete loss of activity
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
25% of maximal activity at pH 6, 90% of maximal activity at pH 8
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
1 mM, slightly stimulates
Rattus norvegicus
Nonidet P-40
stimulates at 0.05%
Rattus norvegicus
octyl glucoside
slight stimulation
Rattus norvegicus
sodium deoxycholate
stimulates at 0.5%
Rattus norvegicus
Triton X-100
0.1%, stimulates
Rattus norvegicus
Tween 20
0.05%, slight stimulation
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Sodium dodecyl sulfate
at concentration 0.5%: 92% loss of activity
Rattus norvegicus
Tween 20
inhibits slightly at 0.5%
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
palmitoyl-CoA
-
Rattus norvegicus
0.046
-
oleoyl-CoA
-
Rattus norvegicus
0.05
-
stearoyl-CoA
-
Rattus norvegicus
0.219
-
myristoyl-CoA
-
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
MgCl2
2 mM, stimulates
Rattus norvegicus
MnCl2
stimulates
Rattus norvegicus
NaF
slight stimulation
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rattus norvegicus
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
?
-
-
-
palmitoyl-CoA + myelin proteolipid protein
Rattus norvegicus
-
CoA + myelin proteolipid O-palmitoylprotein
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain myelin
labeled product also found in vitro in brain tissue slices
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
freezing and thawing causes marked decrease in activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
486083
Rattus norvegicus
?
-
-
-
-
myristoyl-CoA + myelin proteolipid protein
low activity
486083
Rattus norvegicus
CoA + myelin proteolipid O-myristoylprotein
-
-
-
?
oleoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-oleoylprotein
-
-
-
?
palmitoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-palmitoylprotein
-
-
-
?
palmitoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-palmitoylprotein
-
486083
Rattus norvegicus
?
stearoyl-CoA + myelin proteolipid protein
-
486083
Rattus norvegicus
CoA + myelin proteolipid O-stearoylprotein
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
50
45% of maximal acticity at 10C, 75% of maximal activity at 50C
Rattus norvegicus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
75
-
5 min, complete loss of activity
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
25% of maximal activity at pH 6, 90% of maximal activity at pH 8
Rattus norvegicus
Other publictions for EC 2.3.1.100
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486083
Bizzozero
Acylation of endogenous myelin ...
Rattus norvegicus
J. Biol. Chem.
262
2138-2145
1987
6
-
-
-
-
-
2
4
-
3
-
2
-
1
-
-
1
-
-
1
-
1
6
-
1
1
1
-
1
1
-
-
-
-
-
6
-
-
-
-
-
-
-
2
-
4
-
3
-
2
-
-
-
1
-
1
-
1
6
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-