Literature summary for 2.3.1.1 extracted from

Das, U.; Singh, E.; Dharavath, S.; Tiruttani Subhramanyam, U.K.; Pal, R.K.; Vijayan, R.; Menon, S.; Kumar, S.; Gourinath, S.; Srinivasan, A.
Structural insights into the substrate binding mechanism of novel ArgA from Mycobacterium tuberculosis (2019), Int. J. Biol. Macromol., 125, 970-978 .

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures complexed with CoA and product bound N-acetylglutamine and the other complexed with acetyl-CoA and the inhibitor L-arginine at 2.3 and 3.0 A resolution, respectively. The ArgA protomer has a V cleft and a beta bulge. ArgA may also acetylate L-glutamine like L-glutamate. The active site is strongly inhibited by L-arginine resulting in a closed conformation of ArgA. Both L-arginine and N-acetylglutamine occupy the same active site	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

structures complexed with CoA and product bound N-acetylglutamine and the other complexed with acetyl-CoA and the inhibitor L-arginine at 2.3 and 3.0 A resolution, respectively. The ArgA protomer has a V cleft and a beta bulge. ArgA may also acetylate L-glutamine like L-glutamate. The active site is strongly inhibited by L-arginine resulting in a closed conformation of ArgA. Both L-arginine and N-acetylglutamine occupy the same active site

Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
L-arginine	L-arginine does not act as an allosteric inhibitor	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O33289	-	-
Mycobacterium tuberculosis H37Rv	O33289	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-CoA + L-glutamate	-	Mycobacterium tuberculosis	CoA + N-acetyl-L-glutamate	-	?
acetyl-CoA + L-glutamate	-	Mycobacterium tuberculosis H37Rv	CoA + N-acetyl-L-glutamate	-	?
acetyl-CoA + L-glutamine	-	Mycobacterium tuberculosis	CoA + N-acetyl-L-glutamine	-	?
acetyl-CoA + L-glutamine	-	Mycobacterium tuberculosis H37Rv	CoA + N-acetyl-L-glutamine	-	?

Synonyms

Synonyms	Comment	Organism
ArgA	-	Mycobacterium tuberculosis
Rv2747	-	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0012	-	substrate L-glutamate, pH 8.0, temperature not specified in the publication	Mycobacterium tuberculosis	L-arginine
0.00156	-	substrate L-glutamine, pH 8.0, temperature not specified in the publication	Mycobacterium tuberculosis	L-arginine