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Literature summary for 2.3.1.1 extracted from

  • Caldovic, L.; Haskins, N.; Mumo, A.; Majumdar, H.; Pinter, M.; Tuchman, M.; Krufka, A.
    Expression pattern and biochemical properties of zebrafish N-acetylglutamate synthase (2014), PLoS ONE, 9, e85597.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Danio rerio

Inhibitors

Inhibitors Comment Organism Structure
L-arginine partially inhibited by L-arginin Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 acetyl-CoA apparent value, recombinant mature enzyme, pH and temperature not specified in the publication Danio rerio
0.23
-
 acetyl-CoA apparent value, recombinant catalytic domain, pH and temperature not specified in the publication Danio rerio
1.12
-
 L-glutamate apparent value, recombinant catalytic domain, pH and temperature not specified in the publication Danio rerio
1.13
-
 L-glutamate apparent value, recombinant mature enzyme, pH and temperature not specified in the publication Danio rerio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 4 * 52000, catalytic domain, SDS-PAGE Danio rerio
52752
-
 4 * 52752, catalytic domain, calculated from amino acid sequence Danio rerio
55000
-
 4 * 55000, mature enzyme, SDS-PAGE Danio rerio
55498
-
 4 * 55498, mature enzyme, calculated from amino acid sequence Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate Danio rerio
-
 CoA + N-acetyl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity column chromatography Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Danio rerio CoA + N-acetyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 52000, catalytic domain, SDS-PAGE Danio rerio
homotetramer 4 * 52752, catalytic domain, calculated from amino acid sequence Danio rerio
homotetramer 4 * 55000, mature enzyme, SDS-PAGE Danio rerio
homotetramer 4 * 55498, mature enzyme, calculated from amino acid sequence Danio rerio

Synonyms

Synonyms Comment Organism
N-acetylglutamate synthase
-
 Danio rerio
NAGS
-
 Danio rerio

Expression

Organism Comment Expression
Danio rerio the enzyme is expressed between 24 and 48 h post fertilization up

General Information

General Information Comment Organism
physiological function the enzyme plays a role in citrulline and/or arginine biosynthesis during the first day of development and in ammonia detoxification thereafter Danio rerio