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Literature summary for 2.3.1.1 extracted from

  • Min, L.; Jin, Z.; Caldovic, L.; Morizono, H.; Allewell, N.M.; Tuchman, M.; Shi, D.
    Mechanism of allosteric inhibition of N-acetyl-L-glutamate synthase by L-arginine (2009), J. Biol. Chem., 284, 4873-4880.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Neisseria gonorrhoeae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method Neisseria gonorrhoeae

Inhibitors

Inhibitors Comment Organism Structure
L-arginine
-
 Neisseria gonorrhoeae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 acetyl-CoA
-
 Neisseria gonorrhoeae
4.5
-
 L-glutamate
-
 Neisseria gonorrhoeae
6.6
-
 L-glutamate in presence of 0.1 mM L-arginine Neisseria gonorrhoeae
27.5
-
 L-glutamate in presence of 1 mM L-arginine Neisseria gonorrhoeae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000
-
 gel filtration Neisseria gonorrhoeae

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae Q5FAK7
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity and DEAE columns Neisseria gonorrhoeae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4
-
 residual activity at inhibition by L-arginine Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate assay at pH 8.5, 30°C, inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae CoA + N-acetyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
hexamer inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae

Synonyms

Synonyms Comment Organism
N-acetyl-L-glutamate synthase inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae
NAGS
-
 Neisseria gonorrhoeae
ngNAGS
-
 Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Neisseria gonorrhoeae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.19
-
-
 Neisseria gonorrhoeae L-arginine

General Information

General Information Comment Organism
physiological function first step in L-arginine biosynthesis in plants and micro organsims, inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae