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Literature summary for 2.3.1.1 extracted from

  • Sancho-Vaello, E.; Fernandez-Murga, M.L.; Rubio, V.
    Site-directed mutagenesis studies of acetylglutamate synthase delineate the site for the arginine inhibitor (2008), FEBS Lett., 582, 1081-1086.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E269A mutation affects the putative arginine site Pseudomonas aeruginosa
E352A mutation affects the GCN5-related N-acetyltransferase domain Pseudomonas aeruginosa
E352D mutation affects the GCN5-related N-acetyltransferase domain Pseudomonas aeruginosa
G146C corresponding to clinical mutation Pseudomonas aeruginosa
G275A mutation affects the putative arginine site Pseudomonas aeruginosa
G339A mutation affects the GCN5-related N-acetyltransferase domain Pseudomonas aeruginosa
G368A mutation affects the GCN5-related N-acetyltransferase domain Pseudomonas aeruginosa
K199A mutation affects the putative arginine site Pseudomonas aeruginosa
L353V corresponding to clinical mutation Pseudomonas aeruginosa
V358A mutation affects the GCN5-related N-acetyltransferase domain Pseudomonas aeruginosa
Y14A mutation affects the putative arginine site Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
arginine 10 mM, 90% inhibition for wild-type enzyme and mutations affecting the putative arginine site, about 70-80% inhibition for mutations affecting the GCN5-related N-acetyltransferase domain, 98% inhibition for mutant E352D Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
acetyl-CoA mutant G339A Pseudomonas aeruginosa
0.8
-
acetyl-CoA wild-type Pseudomonas aeruginosa
0.9
-
acetyl-CoA mutant E269A Pseudomonas aeruginosa
0.9
-
acetyl-CoA mutant G146C Pseudomonas aeruginosa
0.9
-
acetyl-CoA mutant G275A Pseudomonas aeruginosa
1
-
acetyl-CoA mutant K199A Pseudomonas aeruginosa
1.1
-
acetyl-CoA mutant Y14A Pseudomonas aeruginosa
1.2
-
acetyl-CoA mutant V358A Pseudomonas aeruginosa
1.8
-
acetyl-CoA mutant L353V Pseudomonas aeruginosa
1.9
-
acetyl-CoA mutant G368A Pseudomonas aeruginosa
2.3
-
acetyl-CoA mutant E352A Pseudomonas aeruginosa
2.7
-
acetyl-CoA mutant E352D Pseudomonas aeruginosa
5
-
L-glutamate wild-type Pseudomonas aeruginosa
5.4
-
L-glutamate mutant G275A Pseudomonas aeruginosa
5.6
-
L-glutamate mutant L353V Pseudomonas aeruginosa
6.7
-
L-glutamate mutant K199A Pseudomonas aeruginosa
7.9
-
L-glutamate mutant E269A Pseudomonas aeruginosa
11.8
-
L-glutamate mutant G368A Pseudomonas aeruginosa
11.9
-
L-glutamate mutant Y14A Pseudomonas aeruginosa
12.9
-
L-glutamate mutant G339A Pseudomonas aeruginosa
120
-
L-glutamate mutant G146C Pseudomonas aeruginosa
342
-
L-glutamate mutant V358A Pseudomonas aeruginosa
438
-
L-glutamate mutant E352D Pseudomonas aeruginosa
557
-
L-glutamate mutant E352A Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49070
-
SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P22567
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate assay at pH 9, 37°C Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
?

Subunits

Subunits Comment Organism
hexamer
-
Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
acetylglutamate synthase
-
Pseudomonas aeruginosa
N-acetyl-L-glutamate synthase
-
Pseudomonas aeruginosa
NAGS
-
Pseudomonas aeruginosa
PaNAGS
-
Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function first enzyme of bacterial and plant arginine biosynthesis, activator of urea cycle in animals Pseudomonas aeruginosa