Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E269A | mutation affects the putative arginine site | Pseudomonas aeruginosa |
E352A | mutation affects the GCN5-related N-acetyltransferase domain | Pseudomonas aeruginosa |
E352D | mutation affects the GCN5-related N-acetyltransferase domain | Pseudomonas aeruginosa |
G146C | corresponding to clinical mutation | Pseudomonas aeruginosa |
G275A | mutation affects the putative arginine site | Pseudomonas aeruginosa |
G339A | mutation affects the GCN5-related N-acetyltransferase domain | Pseudomonas aeruginosa |
G368A | mutation affects the GCN5-related N-acetyltransferase domain | Pseudomonas aeruginosa |
K199A | mutation affects the putative arginine site | Pseudomonas aeruginosa |
L353V | corresponding to clinical mutation | Pseudomonas aeruginosa |
V358A | mutation affects the GCN5-related N-acetyltransferase domain | Pseudomonas aeruginosa |
Y14A | mutation affects the putative arginine site | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | 10 mM, 90% inhibition for wild-type enzyme and mutations affecting the putative arginine site, about 70-80% inhibition for mutations affecting the GCN5-related N-acetyltransferase domain, 98% inhibition for mutant E352D | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
acetyl-CoA | mutant G339A | Pseudomonas aeruginosa | |
0.8 | - |
acetyl-CoA | wild-type | Pseudomonas aeruginosa | |
0.9 | - |
acetyl-CoA | mutant E269A | Pseudomonas aeruginosa | |
0.9 | - |
acetyl-CoA | mutant G146C | Pseudomonas aeruginosa | |
0.9 | - |
acetyl-CoA | mutant G275A | Pseudomonas aeruginosa | |
1 | - |
acetyl-CoA | mutant K199A | Pseudomonas aeruginosa | |
1.1 | - |
acetyl-CoA | mutant Y14A | Pseudomonas aeruginosa | |
1.2 | - |
acetyl-CoA | mutant V358A | Pseudomonas aeruginosa | |
1.8 | - |
acetyl-CoA | mutant L353V | Pseudomonas aeruginosa | |
1.9 | - |
acetyl-CoA | mutant G368A | Pseudomonas aeruginosa | |
2.3 | - |
acetyl-CoA | mutant E352A | Pseudomonas aeruginosa | |
2.7 | - |
acetyl-CoA | mutant E352D | Pseudomonas aeruginosa | |
5 | - |
L-glutamate | wild-type | Pseudomonas aeruginosa | |
5.4 | - |
L-glutamate | mutant G275A | Pseudomonas aeruginosa | |
5.6 | - |
L-glutamate | mutant L353V | Pseudomonas aeruginosa | |
6.7 | - |
L-glutamate | mutant K199A | Pseudomonas aeruginosa | |
7.9 | - |
L-glutamate | mutant E269A | Pseudomonas aeruginosa | |
11.8 | - |
L-glutamate | mutant G368A | Pseudomonas aeruginosa | |
11.9 | - |
L-glutamate | mutant Y14A | Pseudomonas aeruginosa | |
12.9 | - |
L-glutamate | mutant G339A | Pseudomonas aeruginosa | |
120 | - |
L-glutamate | mutant G146C | Pseudomonas aeruginosa | |
342 | - |
L-glutamate | mutant V358A | Pseudomonas aeruginosa | |
438 | - |
L-glutamate | mutant E352D | Pseudomonas aeruginosa | |
557 | - |
L-glutamate | mutant E352A | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49070 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P22567 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | assay at pH 9, 37°C | Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
acetylglutamate synthase | - |
Pseudomonas aeruginosa |
N-acetyl-L-glutamate synthase | - |
Pseudomonas aeruginosa |
NAGS | - |
Pseudomonas aeruginosa |
PaNAGS | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | first enzyme of bacterial and plant arginine biosynthesis, activator of urea cycle in animals | Pseudomonas aeruginosa |