Literature summary for 2.3.1.1 extracted from

Davies, A.M.; Tata, R.; Chauviac, F.X.; Sutton, B.J.; Brown, P.R.
Structure of a putative acetyltransferase (PA1377) from Pseudomonas aeruginosa (2008), Acta Crystallogr. Sect. F, 64, 338-342.

Cloned(Commentary)

Cloned (Comment)	Organism
gene PA1377, cloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml protein solution containing 13 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, and 0.1% w/v sodium azide, 18°C, 1-2 days, cryoprotection in 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, 30% v/v glycerol and 0.1% w/v sodium azide, X-ray diffraction structure determination and analysis at 2.25 A resolution	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml protein solution containing 13 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, and 0.1% w/v sodium azide, 18°C, 1-2 days, cryoprotection in 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, 30% v/v glycerol and 0.1% w/v sodium azide, X-ray diffraction structure determination and analysis at 2.25 A resolution

Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I3W7	gene PA1377	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-methionine	pitax is an acetyltransferase and weakly catalyses the acetylation of l-methionine and l-methionine sulfoxide. Pitax belongs to the GCN5-related N-acetyltransferase family and contains all four sequence motifs conserved among family members. The beta4-strand structure in one of these motifs is disrupted, which is believed to affect binding of the substrate that accepts the acetyl group from acetyl-CoA	Pseudomonas aeruginosa	CoA + N-acetyl-L-methionine	-	?
acetyl-CoA + L-methionine sulfoxide	pitax is an acetyltransferase and weakly catalyses the acetylation of l-methionine and l-methionine sulfoxide. Pitax belongs to the GCN5-related N-acetyltransferase family and contains all four sequence motifs conserved among family members. The beta4-strand structure in one of these motifs is disrupted, which is believed to affect binding of the substrate that accepts the acetyl group from acetyl-CoA	Pseudomonas aeruginosa	CoA + N-acetyl-L-methionine sulfoxide	-	?

Subunits

Subunits	Comment	Organism
More	pitax is an alpha/beta-fold protein with an overall structure of two regions of antiparallel beta-sheet, each flanked by alpha-helices	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
More	pitax belongs to the GCN5-related N-acetyltransferase family	Pseudomonas aeruginosa
pitax	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Pseudomonas aeruginosa