Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA- | Homo sapiens |
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA-, overexpression of the preenzyme in insect cells via baculovirus infection system, 2 processed forms with different N-terminal truncations are produced | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the enzyme contains an N-terminal putative mitochondrial targeting sequence | Mus musculus | 5739 | - |
mitochondrion | the enzyme contains an N-terminal putative mitochondrial targeting sequence | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | Mus musculus | - |
CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | Homo sapiens | - |
CoA + N-acetyl-L-glutamate | - |
? | |
additional information | Mus musculus | enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity | ? | - |
? | |
additional information | Homo sapiens | enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | 2 processed forms with different N-terminal truncations are produced, i.e. a long and a short mature form | Mus musculus |
proteolytic modification | 2 processed forms with different N-terminal truncations are produced, i.e. a long and a short mature form | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mus musculus |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Mus musculus | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Homo sapiens | CoA + N-acetyl-L-glutamate | - |
? | |
additional information | enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity | Mus musculus | ? | - |
? | |
additional information | enzyme deficiency causes hyperammonemia, presumably due to loss of carbamoylphosphate synthase I activity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme possesses a carbamate kinase fold and an acyl-CoA N-acyltransferase fold | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
N-acetylglutamate synthase | - |
Mus musculus |
N-acetylglutamate synthase | - |
Homo sapiens |
NAGS | - |
Mus musculus |
NAGS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Mus musculus |
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mus musculus | |
acetyl-CoA | - |
Homo sapiens |