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Literature summary for 2.2.1.8 extracted from

  • Wu, L.; Tong, M.H.; Raab, A.; Fang, Q.; Wang, S.; Kyeremeh, K.; Yu, Y.; Deng, H.
    An unusual metal-bound 4-fluorothreonine transaldolase from Streptomyces sp. MA37 catalyses promiscuous transaldol reactions (2020), Appl. Microbiol. Biotechnol., 104, 3885-3896 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans strain 66 Streptomyces sp. MA37

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.241
-
fluoroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.357
-
glycolaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.358
-
phenylacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.535
-
Isobutyraldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
1.18
-
L-threonine at pH 7.5 and 28°C Streptomyces sp. MA37
1.18
-
L-allo-threonine at pH 7.5 and 28°C Streptomyces sp. MA37
1.35
-
Chloroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
1.453
-
propanal at pH 7.5 and 28°C Streptomyces sp. MA37

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Streptomyces sp. MA37

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + fluoroacetaldehyde Streptomyces sp. MA37
-
acetaldehyde + 4-fluoro-L-threonine
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. MA37
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind affinity column chromatography and gel filtration Streptomyces sp. MA37

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine + fluoroacetaldehyde very low activity Streptomyces sp. MA37 ? + 4-fluoro-L-threonine
-
?
L-serine + fluoroacetaldehyde very low activity Streptomyces sp. MA37 ? + 4-fluoro-L-threonine
-
?
L-threonine + chloroacetaldehyde
-
Streptomyces sp. MA37 acetaldehyde + 4-chloro-L-threonine
-
?
L-threonine + fluoroacetaldehyde
-
Streptomyces sp. MA37 acetaldehyde + 4-fluoro-L-threonine
-
?
L-threonine + glycolaldehyde
-
Streptomyces sp. MA37 acetaldehyde + 4-hydroxyl-L-threonine
-
?
L-threonine + isobutyraldehyde
-
Streptomyces sp. MA37 acetaldehyde + 4-isobutyl-L-threonine
-
?
L-threonine + phenylacetaldehyde
-
Streptomyces sp. MA37 acetaldehyde + 4-phenyl-L-threonine
-
?
L-threonine + propanal
-
Streptomyces sp. MA37 acetaldehyde + 4-methyl-L-threonine
-
?
additional information no activity with D-allo-threonine, D-threonine or glycine or L-alanine or L-leucine Streptomyces sp. MA37 ?
-
-

Subunits

Subunits Comment Organism
? x * 72100, SDS-PAGE Streptomyces sp. MA37

Synonyms

Synonyms Comment Organism
4-fluorothreonine transaldolase
-
Streptomyces sp. MA37
4-FTase
-
Streptomyces sp. MA37
FTase
-
Streptomyces sp. MA37

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
L-allo-threonine at pH 7.5 and 28°C Streptomyces sp. MA37
0.01
-
phenylacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.013
-
Isobutyraldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.02
-
propanal at pH 7.5 and 28°C Streptomyces sp. MA37
0.024
-
glycolaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.024
-
Chloroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.03
-
fluoroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.052
-
L-threonine at pH 7.5 and 28°C Streptomyces sp. MA37

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Streptomyces sp. MA37

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.006
-
L-allo-threonine at pH 7.5 and 28°C Streptomyces sp. MA37
0.014
-
propanal at pH 7.5 and 28°C Streptomyces sp. MA37
0.0176
-
Chloroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.0254
-
Isobutyraldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.02725
-
phenylacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.0442
-
L-threonine at pH 7.5 and 28°C Streptomyces sp. MA37
0.0682
-
glycolaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37
0.1246
-
fluoroacetaldehyde at pH 7.5 and 28°C Streptomyces sp. MA37