Cloned (Comment) | Organism |
---|---|
expressed in Streptomyces lividans strain 66 | Streptomyces sp. MA37 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.241 | - |
fluoroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.357 | - |
glycolaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.358 | - |
phenylacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.535 | - |
Isobutyraldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
1.18 | - |
L-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
1.18 | - |
L-allo-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
1.35 | - |
Chloroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
1.453 | - |
propanal | at pH 7.5 and 28°C | Streptomyces sp. MA37 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Streptomyces sp. MA37 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + fluoroacetaldehyde | Streptomyces sp. MA37 | - |
acetaldehyde + 4-fluoro-L-threonine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. MA37 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Bind affinity column chromatography and gel filtration | Streptomyces sp. MA37 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-threonine + fluoroacetaldehyde | very low activity | Streptomyces sp. MA37 | ? + 4-fluoro-L-threonine | - |
? | |
L-serine + fluoroacetaldehyde | very low activity | Streptomyces sp. MA37 | ? + 4-fluoro-L-threonine | - |
? | |
L-threonine + chloroacetaldehyde | - |
Streptomyces sp. MA37 | acetaldehyde + 4-chloro-L-threonine | - |
? | |
L-threonine + fluoroacetaldehyde | - |
Streptomyces sp. MA37 | acetaldehyde + 4-fluoro-L-threonine | - |
? | |
L-threonine + glycolaldehyde | - |
Streptomyces sp. MA37 | acetaldehyde + 4-hydroxyl-L-threonine | - |
? | |
L-threonine + isobutyraldehyde | - |
Streptomyces sp. MA37 | acetaldehyde + 4-isobutyl-L-threonine | - |
? | |
L-threonine + phenylacetaldehyde | - |
Streptomyces sp. MA37 | acetaldehyde + 4-phenyl-L-threonine | - |
? | |
L-threonine + propanal | - |
Streptomyces sp. MA37 | acetaldehyde + 4-methyl-L-threonine | - |
? | |
additional information | no activity with D-allo-threonine, D-threonine or glycine or L-alanine or L-leucine | Streptomyces sp. MA37 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 72100, SDS-PAGE | Streptomyces sp. MA37 |
Synonyms | Comment | Organism |
---|---|---|
4-fluorothreonine transaldolase | - |
Streptomyces sp. MA37 |
4-FTase | - |
Streptomyces sp. MA37 |
FTase | - |
Streptomyces sp. MA37 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
L-allo-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.01 | - |
phenylacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.013 | - |
Isobutyraldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.02 | - |
propanal | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.024 | - |
glycolaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.024 | - |
Chloroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.03 | - |
fluoroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.052 | - |
L-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Streptomyces sp. MA37 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
L-allo-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.014 | - |
propanal | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.0176 | - |
Chloroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.0254 | - |
Isobutyraldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.02725 | - |
phenylacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.0442 | - |
L-threonine | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.0682 | - |
glycolaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 | |
0.1246 | - |
fluoroacetaldehyde | at pH 7.5 and 28°C | Streptomyces sp. MA37 |