Protein Variants | Comment | Organism |
---|---|---|
R420A | the mutation has no apparent effect on the affinities of nitroso substrates in C-N bond formation | Escherichia coli |
R478A | the mutation has no apparent effect on the affinities of nitroso substrates in C-N bond formation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl-2-nitrosobenzene | weak inhibitory activity | Escherichia coli | |
1-nitrosonaphthalen-2-ol | weak inhibitory activity | Escherichia coli | |
3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | weak inhibitory activity | Escherichia coli | |
6-bromo-1-nitrosonaphthalen-2-ol | weak inhibitory activity | Escherichia coli | |
6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | weak inhibitory activity | Escherichia coli | |
7-methoxy-1-nitrosonaphthalen-2-ol | weak inhibitory activity | Escherichia coli | |
benzylacetylphosphonate | competitive inhibitor | Escherichia coli | |
N,N-dimethyl-4-nitrosoaniline | weak inhibitory activity | Escherichia coli | |
nitrosobenzene | weak inhibitory activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | at pH 8.0 and 37°C | Escherichia coli | |
0.024 | - |
6-bromo-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.041 | - |
1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.054 | - |
N,N-dimethyl-4-nitrosoaniline | at pH 8.0 and 37°C | Escherichia coli | |
0.063 | - |
7-methoxy-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.099 | - |
1-methyl-2-nitrosobenzene | at pH 8.0 and 37°C | Escherichia coli | |
0.387 | - |
3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | at pH 8.0 and 37°C | Escherichia coli | |
0.512 | - |
2-hydroxy-4,6-dinitrobenzaldehyde | at pH 8.0 and 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde 3-phosphate | Escherichia coli | - |
1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77488 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + 1-methyl-2-nitrosobenzene | - |
Escherichia coli | N-hydroxy-N-(2-methylphenyl)acetamide + CO2 | - |
? | |
pyruvate + 1-nitrosonaphthalen-2-ol | - |
Escherichia coli | N-hydroxy-N-(2-hydroxynaphthalen-1-yl)acetamide + CO2 | - |
? | |
pyruvate + 2-hydroxy-4,6-dinitrobenzaldehyde | - |
Escherichia coli | ? + CO2 | - |
? | |
pyruvate + 3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | - |
Escherichia coli | N-[3,5-dimethyl-1-(propan-2-yl)-1H-pyrazol-4-yl]-N-hydroxyacetamide + CO2 | - |
? | |
pyruvate + 6-bromo-1-nitrosonaphthalen-2-ol | - |
Escherichia coli | N-(6-bromo-2-hydroxynaphthalen-1-yl)-N-hydroxyacetamide + CO2 | - |
? | |
pyruvate + 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | - |
Escherichia coli | N-hydroxy-N-(2-hydroxy-6-sulfamoylnaphthalen-1-yl)acetamide + CO2 | - |
? | |
pyruvate + 7-methoxy-1-nitrosonaphthalen-2-ol | - |
Escherichia coli | N-hydroxy-N-(2-hydroxy-7-methoxynaphthalen-1-yl)acetamide + CO2 | - |
? | |
pyruvate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
pyruvate + N,N-dimethyl-4-nitrosoaniline | - |
Escherichia coli | N-[4-(dimethylamino)phenyl]-N-hydroxyacetamide + CO2 | - |
? | |
pyruvate + nitrosobenzene | - |
Escherichia coli | ? + CO2 | - |
? | |
pyruvate + nitrosobenzene | - |
Escherichia coli | N-hydroxy-N-phenylacetamide + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose 5-phosphate synthase | - |
Escherichia coli |
DXP synthase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2-hydroxy-4,6-dinitrobenzaldehyde | at pH 8.0 and 37°C | Escherichia coli | |
0.015 | - |
N,N-dimethyl-4-nitrosoaniline | at pH 8.0 and 37°C | Escherichia coli | |
0.018 | - |
1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.02 | - |
6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | at pH 8.0 and 37°C | Escherichia coli | |
0.022 | - |
7-methoxy-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.023 | - |
3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | at pH 8.0 and 37°C | Escherichia coli | |
0.033 | - |
6-bromo-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.6 | - |
1-methyl-2-nitrosobenzene | at pH 8.0 and 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0104 | - |
benzylacetylphosphonate | at pH 8.0 and 37°C | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.208 | - |
at pH 8.0 and 37°C | Escherichia coli | nitrosobenzene | |
0.291 | - |
at pH 8.0 and 37°C | Escherichia coli | 1-methyl-2-nitrosobenzene | |
0.354 | - |
at pH 8.0 and 37°C | Escherichia coli | 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | |
0.522 | - |
at pH 8.0 and 37°C | Escherichia coli | 6-bromo-1-nitrosonaphthalen-2-ol | |
0.844 | - |
at pH 8.0 and 37°C | Escherichia coli | N,N-dimethyl-4-nitrosoaniline | |
1.065 | - |
at pH 8.0 and 37°C | Escherichia coli | 1-nitrosonaphthalen-2-ol | |
2 | - |
IC50 above 2.0 mM, at pH 8.0 and 37°C | Escherichia coli | 7-methoxy-1-nitrosonaphthalen-2-ol | |
2 | - |
IC50 above 2.0 mM, at pH 8.0 and 37°C | Escherichia coli | 3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
0.06 | - |
3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | at pH 8.0 and 37°C | Escherichia coli | |
0.28 | - |
N,N-dimethyl-4-nitrosoaniline | at pH 8.0 and 37°C | Escherichia coli | |
0.35 | - |
7-methoxy-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
1.1 | - |
6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | at pH 8.0 and 37°C | Escherichia coli | |
1.3 | - |
6-bromo-1-nitrosonaphthalen-2-ol | at pH 8.0 and 37°C | Escherichia coli | |
6 | - |
1-methyl-2-nitrosobenzene | at pH 8.0 and 37°C | Escherichia coli |