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Literature summary for 2.2.1.6 extracted from

  • Zheng, P.; Sun, X.; Guo, L.; Shen, J.
    Cloning, expression, and characterization of an acetolactate synthase (ALS) gene from Anabaena azotica (2015), Process Biochem., 50, 1349-1356 .
No PubMed abstract available
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Anabaena azotica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Anabaena azotica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Anabaena azotica
-
 2-acetolactate + CO2
-
 ?
2 pyruvate Anabaena azotica FACHB-686
-
 2-acetolactate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena azotica
-
-
-
Anabaena azotica FACHB-686
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA-Sefinose column chromatography Anabaena azotica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
 purified enzyme, at pH 7.4 and 37°C Anabaena azotica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Anabaena azotica 2-acetolactate + CO2
-
 ?
2 pyruvate
-
 Anabaena azotica FACHB-686 2-acetolactate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 59200, calculated from amino acid sequence and SDS-PAGE Anabaena azotica

Synonyms

Synonyms Comment Organism
aceto-hydroxy acid synthase
-
 Anabaena azotica
AHAS
-
 Anabaena azotica
ALS
-
 Anabaena azotica

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Anabaena azotica
thiamine diphosphate
-
 Anabaena azotica