Cloned (Comment) | Organism |
---|---|
expressed in Oryza sativa subsp. japonica variety JD164 and Escherichia coli Rosetta cells | Oryza sativa Indica Group |
Protein Variants | Comment | Organism |
---|---|---|
S627N | the mutation confers tolerance against imidazolinone herbicides, including imazethapyr and imazamox. The mutant is tolerant to imidazolinone but the catalytic efficiency of the mutated enzyme decreases in its presence. Moreover, the activity of the mutated enzyme decreases more in the presence of imazethapyr than in the presence of imazamox | Oryza sativa Indica Group |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bispyribac | - |
Oryza sativa Indica Group | |
chlorsulfuron | - |
Oryza sativa Indica Group | |
imazamox | - |
Oryza sativa Indica Group | |
imazethapyr | - |
Oryza sativa Indica Group |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.5 | - |
pyruvate | wild type enzyme, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
8.8 | - |
pyruvate | mutant enzyme S627N, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
9 | - |
pyruvate | mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
11.4 | - |
pyruvate | mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C | Oryza sativa Indica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Oryza sativa Indica Group | - |
2-acetolactate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Indica Group | A5X300 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Oryza sativa Indica Group | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, mature enzyme, SDS-PAGE | Oryza sativa Indica Group |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxy acid synthase | - |
Oryza sativa Indica Group |
acetolactate synthase | - |
Oryza sativa Indica Group |
AHAS | - |
Oryza sativa Indica Group |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
pyruvate | mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
4.1 | - |
pyruvate | mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
4.5 | - |
pyruvate | wild type enzyme, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
4.6 | - |
pyruvate | mutant enzyme S627N, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
8.8 | - |
pyruvate | wild type enzyme, at pH 7.5 and 20°C | Oryza sativa Indica Group |
Organism | Comment | Expression |
---|---|---|
Oryza sativa Indica Group | the mutant enzyme S627N mRNA level is increased after imazethapyr or imazamox application | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
pyruvate | wild type enzyme, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
0.46 | - |
pyruvate | mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
0.52 | - |
pyruvate | mutant enzyme S627N, at pH 7.5 and 20°C | Oryza sativa Indica Group | |
11.4 | - |
pyruvate | mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C | Oryza sativa Indica Group |