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Literature summary for 2.2.1.6 extracted from

  • Garcia, M.D.; Wang, J.G.; Lonhienne, T.; Guddat, L.W.
    Crystal structure of plant acetohydroxyacid synthase, the target for several commercial herbicides (2017), FEBS J., 284, 2037-2051 .
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with inhibitors, hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 0.1 M CHES and 0.1-0.2 M lithium sulfate Saccharomyces cerevisiae
free enzyme and in complex with inhibitors, hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 0.1 M CHES and 0.1-0.2 M lithium sulfate Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
bispyribac-sodium
-
 Arabidopsis thaliana
bispyribac-sodium
-
 Saccharomyces cerevisiae
chlorimuron ethyl
-
 Arabidopsis thaliana
chlorimuron ethyl
-
 Saccharomyces cerevisiae
chlorsulfuron
-
 Arabidopsis thaliana
chlorsulfuron
-
 Saccharomyces cerevisiae
florasulam
-
 Arabidopsis thaliana
florasulam
-
 Saccharomyces cerevisiae
imazapyr
-
 Arabidopsis thaliana
imazapyr
-
 Saccharomyces cerevisiae
imazaquin
-
 Arabidopsis thaliana
imazaquin
-
 Saccharomyces cerevisiae
imazethapyr
-
 Arabidopsis thaliana
imazethapyr
-
 Saccharomyces cerevisiae
thiencarbazone methyl
-
 Arabidopsis thaliana
thiencarbazone methyl
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Saccharomyces cerevisiae
-
 2-acetolactate + CO2
-
 ?
2 pyruvate Arabidopsis thaliana
-
 2-acetolactate + CO2
-
 ?
2-oxobutyrate + pyruvate Saccharomyces cerevisiae
-
 (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?
2-oxobutyrate + pyruvate Arabidopsis thaliana
-
 (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P17597
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and S-200 HR gel filtration Saccharomyces cerevisiae
Ni-NTA resin column chromatography and S-200 HR gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Saccharomyces cerevisiae 2-acetolactate + CO2
-
 ?
2 pyruvate
-
 Arabidopsis thaliana 2-acetolactate + CO2
-
 ?
2-oxobutyrate + pyruvate
-
 Saccharomyces cerevisiae (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?
2-oxobutyrate + pyruvate
-
 Arabidopsis thaliana (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Saccharomyces cerevisiae
homotetramer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase
-
 Saccharomyces cerevisiae
acetohydroxyacid synthase
-
 Arabidopsis thaliana
AHAS
-
 Saccharomyces cerevisiae
AHAS
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Saccharomyces cerevisiae
FAD
-
 Arabidopsis thaliana
thiamine diphosphate
-
 Saccharomyces cerevisiae
thiamine diphosphate
-
 Arabidopsis thaliana