Literature summary for 2.2.1.6 extracted from

Huo, Y.; Zhan, Y.; Wang, Q.; Li, S.; Yang, S.; Nomura, C.T.; Wang, C.; Chen, S.
Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02 enzymatic properties and efficient functions for acetoin/butanediol and L-valine biosynthesis (2018), Bioprocess Biosyst. Eng., 41, 87-96 .

Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus licheniformis

Inhibitors

Inhibitors	Comment	Organism	Structure
L-isoleucine	-	Bacillus licheniformis
L-leucine	-	Bacillus licheniformis
L-valine	-	Bacillus licheniformis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.96	-	pyruvate	at pH 6.5 and 37°C	Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 pyruvate	Bacillus licheniformis	-	2-acetolactate + CO2	-	?
2 pyruvate	Bacillus licheniformis WX-02	-	2-acetolactate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-
Bacillus licheniformis WX-02	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
35.4	-	at pH 6.5 and 37°C	Bacillus licheniformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 pyruvate	-	Bacillus licheniformis	2-acetolactate + CO2	-	?
2 pyruvate	-	Bacillus licheniformis WX-02	2-acetolactate + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 63000, SDS-PAGE	Bacillus licheniformis

Synonyms

Synonyms	Comment	Organism
acetolactate synthase	-	Bacillus licheniformis
AlsS	-	Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	at pH 6.5	Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	the enzyme shows more than 50% activity between 20 and 50°C. The activity at 60°C is less than 40% and then completely decreases at 70-80°C	Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
514	-	pyruvate	at pH 6.5 and 37°C	Bacillus licheniformis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Bacillus licheniformis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	7.5	the enzyme exhibits more than 60% activity in the range of pH 5.5-7.5	Bacillus licheniformis

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Bacillus licheniformis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00273	-	L-valine	at pH 6.5 and 37°C	Bacillus licheniformis
0.0119	-	L-leucine	at pH 6.5 and 37°C	Bacillus licheniformis
0.19	-	L-isoleucine	at pH 6.5 and 37°C	Bacillus licheniformis

General Information

General Information	Comment	Organism
malfunction	the enzyme-deficient strain cannot produce acetoin, 2,3-butanediol, and L-valine	Bacillus licheniformis
metabolism	enzyme overexpression can effectively improve acetoin/2,3-butanediol and L-valine production in Bacillus licheniformis	Bacillus licheniformis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)