Cloned (Comment) | Organism |
---|---|
gene yerE, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Yersinia pseudotuberculosis | YerE might posses the ability to activate non-sugar ketones for cross-benzoin condensations, performing enzymatic aldehyde-ketone cross-benzoin condensations | ? | - |
? | |
additional information | Yersinia pseudotuberculosis O:VI | YerE might posses the ability to activate non-sugar ketones for cross-benzoin condensations, performing enzymatic aldehyde-ketone cross-benzoin condensations | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pseudotuberculosis | G4WJC8 | gene yerE | - |
Yersinia pseudotuberculosis O:VI | G4WJC8 | gene yerE | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, to homogeneity | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(1,3-benzodioxol-5-yloxy)propan-2-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
1-(1,3-benzodioxol-5-yloxy)propan-2-one + pyruvate | - |
Yersinia pseudotuberculosis O:VI | ? + CO2 | - |
? | |
1-(2-bromo-5-hydroxyphenoxy)propan-2-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
1-(naphthalen-2-yloxy)propan-2-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
1-(phenylsulfanyl)propan-2-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
1-phenoxypropan-2-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
2 pyruvate | - |
Yersinia pseudotuberculosis | (S)-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Yersinia pseudotuberculosis O:VI | (S)-acetolactate + CO2 | - |
? | |
2-chlorobenzaldehyde + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
2-oxopropyl 4-bromobenzoate + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
3,4-dihydronaphthalen-2(1H)-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
4-hydroxybenzaldehyde + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
cyclohexane-1,2-dione + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
cyclohexanone + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
dihydro-2H-pyran-3(4H)-one + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
ethyl 3-oxobutanoate + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
hexan-3,4-dione + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
methylpyruvate + pyruvate | - |
Yersinia pseudotuberculosis | ? + CO2 | - |
? | |
additional information | YerE might posses the ability to activate non-sugar ketones for cross-benzoin condensations, performing enzymatic aldehyde-ketone cross-benzoin condensations | Yersinia pseudotuberculosis | ? | - |
? | |
additional information | asymmetric intermolecular crossed aldehyde-ketone condensation through enzymatic carboligation reaction. Carboligation reactions catalyzed by the ThDP-dependent enzyme YerE with pyruvate and an acceptor substrate can lead to tertiary alcohols, (R)-phenylacetylcarbinol derivatives, (S )-acetolactate, and (S)-acetoin, overview | Yersinia pseudotuberculosis | ? | - |
? | |
additional information | YerE might posses the ability to activate non-sugar ketones for cross-benzoin condensations, performing enzymatic aldehyde-ketone cross-benzoin condensations | Yersinia pseudotuberculosis O:VI | ? | - |
? | |
additional information | asymmetric intermolecular crossed aldehyde-ketone condensation through enzymatic carboligation reaction. Carboligation reactions catalyzed by the ThDP-dependent enzyme YerE with pyruvate and an acceptor substrate can lead to tertiary alcohols, (R)-phenylacetylcarbinol derivatives, (S )-acetolactate, and (S)-acetoin, overview | Yersinia pseudotuberculosis O:VI | ? | - |
? | |
pyruvate + benzaldehyde | asymmetric benzoin condensation between benzaldehyde and pyruvate | Yersinia pseudotuberculosis | (R)-phenylacetylcarbinol + CO2 | - |
? | |
pyruvate + benzaldehyde | asymmetric benzoin condensation between benzaldehyde and pyruvate | Yersinia pseudotuberculosis O:VI | (R)-phenylacetylcarbinol + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Yersinia pseudotuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Yersinia pseudotuberculosis |