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Literature summary for 2.2.1.6 extracted from

  • Lang, Z.F.; Shen, J.J.; Cai, S.; Zhang, J.; He, J.; Li, S.P.
    Expression, characterization, and site-directed mutation of a multiple herbicide-resistant acetohydroxyacid synthase (rAHAS) from Pseudomonas sp. Lm10 (2011), Curr. Microbiol., 63, 145-150.
    View publication on PubMed
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information cloning of a herbicide-resistant acetohydroxyacid synthase gene from Pseudomonas sp. Lm10. Sequence analysis shows that the regulatory subunit of the resisitant enzyme is identical to that of Pseudomonas putida KT2440, whereas six mutations are found in the catalytic subunit, i.e. resistant AHAS/sensitive AHAS: H134N, A135P, S136T, I210V, F264Y, and S486W Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
flumetsulam herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 6.5fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
imazethapyr herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 12.6fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
L-valine
-
 Pseudomonas sp.
metsulfuron-methyl herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 56fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
pyriminobac-methyl herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 9.2fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.5
-
 pyruvate pH 7.5, 37°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km value 0.00012 mM Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, SDS-PAGE, catalytic subunit Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. G0ZSE6 catalytic subunit
-
Pseudomonas sp. Lm10 G0ZSE6 catalytic subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.5
-
 pH 7.5, 37°C Pseudomonas sp.

Storage Stability

Storage Stability Organism
-70°C, 3 weeks, loss of 5% of activity Pseudomonas sp.
4°C, 3 weeks, loss of 10% of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Pseudomonas sp. 2-acetolactate + CO2
-
 ?
2 pyruvate
-
 Pseudomonas sp. Lm10 2-acetolactate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE, catalytic subunit Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD Km value 0.00033 mM Pseudomonas sp.
thiamine diphosphate Km value 0.0092 mM Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.036
-
 flumetsulam pH 7.5, 37°C Pseudomonas sp.
0.065
-
 pyriminobac-methyl pH 7.5, 37°C Pseudomonas sp.
0.105
-
 L-valine pH 7.5, 37°C Pseudomonas sp.
0.121
-
 imazethapyr pH 7.5, 37°C Pseudomonas sp.
0.201
-
 metsulfuron-methyl pH 7.5, 37°C Pseudomonas sp.