Literature summary for 2.2.1.6 extracted from

Kaplun, A.; Vyazmensky, M.; Zherdev, Y.; Belenky, I.; Slutzker, A.; Mendel, S.; Barak, Z.; Chipman, D.M.; Shaanan, B.
Structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from Escherichia coli (2006), J. Mol. Biol., 357, 951-963.

Cloned(Commentary)

Cloned (Comment)	Organism
gene ilvH, encoding the regulatory subunit SSU, expression of wild-type and mutants, and of the selenomethionine variant	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled isozyme AHAS III in complex with valine, hanging drop vapour diffusion method, room temperature, 0.0036 ml of protein solution containing 10-25 mg/ml protein and 0.5 M MgCl2, is mixed with reservoir solution containing 30-40% PEG 400, 0.4-0.6 M MgCl2, 100 mM Tris-HCl, pH 8.5, tetragonal or orthorhombic crystals, X-ray diffraction structure determination and analysis at 1.75-2.5 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant wild-type and selenomethionine-labeled isozyme AHAS III in complex with valine, hanging drop vapour diffusion method, room temperature, 0.0036 ml of protein solution containing 10-25 mg/ml protein and 0.5 M MgCl2, is mixed with reservoir solution containing 30-40% PEG 400, 0.4-0.6 M MgCl2, 100 mM Tris-HCl, pH 8.5, tetragonal or orthorhombic crystals, X-ray diffraction structure determination and analysis at 1.75-2.5 A resolution

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
A36V	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
G14A	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
G14D	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
L131R	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
L16A	site-directed mutagenesis of the regulatory subunit, the mutant shows increased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
L9A	site-directed mutagenesis of the regulatory subunit, the mutant shows slightly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
L9H	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
L9V	site-directed mutagenesis of the regulatory subunit, the mutant shows slightly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
additional information	the truncated mutant DELTA80 is resistant to inhibition by valine	Escherichia coli
N11A	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
N11D	site-directed mutagenesis of the regulatory subunit, the mutant shows highly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
N11H	site-directed mutagenesis of the regulatory subunit, the mutant shows highly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
N29D	site-directed mutagenesis of the regulatory subunit, the mutant shows highly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
N29H	site-directed mutagenesis of the regulatory subunit, the mutant shows highly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
T34C	site-directed mutagenesis of the regulatory subunit, the mutant shows decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
T34I	site-directed mutagenesis of the regulatory subunit, the mutant shows highly decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
T47C	site-directed mutagenesis of the regulatory subunit, the mutant shows decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli
V153D	site-directed mutagenesis of the regulatory subunit, the mutant is resistant to inhibition by valine	Escherichia coli
V35A	site-directed mutagenesis of the regulatory subunit, the mutant shows decreased sensitivity to valine inhibition compared to the wild-type subunit	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
valine	binding site structure, inhibition mechanism	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates, binding structure at the regulatory subunit	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate	Escherichia coli	the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine	2-acetolactate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	isozyme III	-

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution of the holoenzyme from recombinant small and large subunits, not highly dependent on MgCl2, but when no extraneous magnesium is added the enzyme does not reach its full potential for activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate	-	Escherichia coli	2-acetolactate + CO2	-	?
pyruvate	the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine	Escherichia coli	2-acetolactate + CO2	-	?

Subunits

Subunits	Comment	Organism
More	enzymes in the AHAS family generally consist of regulatory and catalytic subunits, subunit composition, dimeric structure analysis of the regulatory subunit of isozyme AHAS III, overview	Escherichia coli

Synonyms

Synonyms	Comment	Organism
acetohydroxy acid synthase	-	Escherichia coli
AHAS	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli
thiamine diphosphate	-	Escherichia coli